Abstract：
Photosynthesis plays a vital role in acclimating to and mitigating climate change, providing food and energy security for a population that is constantly growing, and achieving an economy with zero carbon emissions. A thorough comprehension of the dynamics of photosynthesis, including its molecular regulatory network and limitations, is essential for utilizing it as a tool to boost plant growth, enhance crop yields, and support the production of plant biomass for carbon storage. Photorespiration constrains photosynthetic efficiency and contributes significantly to carbon loss. Therefore, modulating or circumventing photorespiration presents opportunities to enhance photosynthetic efficiency. Over the past eight decades, substantial progress has been made in elucidating the molecular basis of photosynthesis, photorespiration, and the key regulatory mechanisms involved, beginning with the discovery of the canonical Calvin-Benson-Bassham cycle. Advanced chromatographic and mass spectrometric technologies have allowed a comprehensive analysis of the metabolite patterns associated with photosynthesis, contributing to a deeper understanding of its regulation. In this review, we summarize the results of metabolomics studies that shed light on the molecular intricacies of photosynthetic metabolism. We also discuss the methodological requirements essential for effective analysis of photosynthetic metabolism, highlighting the value of this technology in supporting strategies aimed at enhancing photosynthesis.
摘要：
光合作用在适应和减缓气候变化中起着至关重要的作用。为不断增长的人口提供粮食和能源安全，实现零碳排放的经济。彻底理解光合作用的动力学，包括其分子调控网络和局限性，对于利用它作为促进植物生长的工具至关重要，提高作物产量，并支持用于碳储存的植物生物质的生产。光呼吸限制了光合效率，并显着导致碳损失。因此,调节或规避光呼吸提供了增强光合作用效率的机会。在过去的八十年中，在阐明光合作用的分子基础方面取得了重大进展，光呼吸,以及所涉及的关键监管机制，从经典卡尔文-本森-巴斯舍姆循环的发现开始。先进的色谱和质谱技术允许对与光合作用相关的代谢物模式进行全面分析，有助于对其监管有更深入的了解。在这次审查中,我们总结了代谢组学研究的结果，这些研究揭示了光合代谢的分子复杂性。我们还讨论了有效分析光合代谢所必需的方法学要求，强调这项技术在支持旨在增强光合作用的策略方面的价值。
