Abstract：
OBJECTIVE: Extensive consumption of alcohol during pregnancy can lead to severe complications for the unborn child. Carbohydrate-deficient transferrin (CDT) levels in serum have become a common biomarker for excessive alcohol intake. However, during pregnancy CDT levels can rise to levels above commonly used cut-off values, for reasons unrelated to alcohol intake. The aim of this study is to investigate the changes in CDT values during pregnancy and to determine accurate, trimester dependent reference intervals.
METHODS: 439 serum samples of 147 healthy pregnant women were obtained for trimester 1, 2, 3, and post-partum and were analysed by high-performance liquid chromatography (HPLC) and an N-Latex immunonephelometric assay. New trimester-specific reference intervals were established.
RESULTS: This study demonstrates there is a trimester-dependent increase of %CDT, as up to 39.4% of the population exceeded the previously established upper reference limit of 1.7%. In our study the estimated upper reference limit for %DST/%CDT were 1.55%, 1.96%, 2.05% and 1.35% for trimester 1, 2, 3 and post-partum for the HPLC-method and 2.02%, 2.19%, 2.19% and 1.96% for the N-Latex immunoassay.
CONCLUSIONS: We demonstrate that CDT levels rise during pregnancy. The magnitude of the increase is method-dependent and needs to be taken into account. We have established method- and trimester-specific reference intervals to prevent false-positive results in alcohol abuse screening tests during pregnancy.
METHODS: 439 serum samples of 147 healthy pregnant women were obtained for trimester 1, 2, 3, and post-partum and were analysed by high-performance liquid chromatography (HPLC) and an N-Latex immunonephelometric assay. New trimester-specific reference intervals were established.
RESULTS: This study demonstrates there is a trimester-dependent increase of %CDT, as up to 39.4% of the population exceeded the previously established upper reference limit of 1.7%. In our study the estimated upper reference limit for %DST/%CDT were 1.55%, 1.96%, 2.05% and 1.35% for trimester 1, 2, 3 and post-partum for the HPLC-method and 2.02%, 2.19%, 2.19% and 1.96% for the N-Latex immunoassay.
CONCLUSIONS: We demonstrate that CDT levels rise during pregnancy. The magnitude of the increase is method-dependent and needs to be taken into account. We have established method- and trimester-specific reference intervals to prevent false-positive results in alcohol abuse screening tests during pregnancy.
摘要：
目的：怀孕期间大量饮酒会导致未出生婴儿的严重并发症。血清中碳水化合物缺乏的转铁蛋白（CDT）水平已成为过量饮酒的常见生物标志物。然而,在怀孕期间，CDT水平可以上升到高于常用临界值的水平，与酒精摄入无关的原因。这项研究的目的是调查怀孕期间CDT值的变化，并确定准确的，三个月依赖性参考间隔。
方法：获取147名健康孕妇的1、2、3个月和产后的439份血清样本，并通过高效液相色谱法（HPLC）和N-Latex免疫比浊法进行分析。建立了新的特定于三个月的参考间隔。
结果：这项研究表明，CDT百分比呈三个月依赖性增加，多达39.4%的人口超过了先前确定的1.7%的参考上限。在我们的研究中，%DST/%CDT的估计参考上限为1.55%，1.96%,2.05％和1.35％的三个月1、2、3和产后的HPLC方法和2.02％，2.19%，N-Latex免疫测定为2.19%和1.96%。
结论：我们证明了妊娠期间CDT水平升高。增加的幅度取决于方法，需要考虑。我们已经建立了方法和特定于三个月的参考间隔，以防止怀孕期间酒精滥用筛查测试中的假阳性结果。
方法：获取147名健康孕妇的1、2、3个月和产后的439份血清样本，并通过高效液相色谱法（HPLC）和N-Latex免疫比浊法进行分析。建立了新的特定于三个月的参考间隔。
结果：这项研究表明，CDT百分比呈三个月依赖性增加，多达39.4%的人口超过了先前确定的1.7%的参考上限。在我们的研究中，%DST/%CDT的估计参考上限为1.55%，1.96%,2.05％和1.35％的三个月1、2、3和产后的HPLC方法和2.02％，2.19%，N-Latex免疫测定为2.19%和1.96%。
结论：我们证明了妊娠期间CDT水平升高。增加的幅度取决于方法，需要考虑。我们已经建立了方法和特定于三个月的参考间隔，以防止怀孕期间酒精滥用筛查测试中的假阳性结果。
