METHODS: 439 serum samples of 147 healthy pregnant women were obtained for trimester 1, 2, 3, and post-partum and were analysed by high-performance liquid chromatography (HPLC) and an N-Latex immunonephelometric assay. New trimester-specific reference intervals were established.
RESULTS: This study demonstrates there is a trimester-dependent increase of %CDT, as up to 39.4% of the population exceeded the previously established upper reference limit of 1.7%. In our study the estimated upper reference limit for %DST/%CDT were 1.55%, 1.96%, 2.05% and 1.35% for trimester 1, 2, 3 and post-partum for the HPLC-method and 2.02%, 2.19%, 2.19% and 1.96% for the N-Latex immunoassay.
CONCLUSIONS: We demonstrate that CDT levels rise during pregnancy. The magnitude of the increase is method-dependent and needs to be taken into account. We have established method- and trimester-specific reference intervals to prevent false-positive results in alcohol abuse screening tests during pregnancy.
方法:获取147名健康孕妇的1、2、3个月和产后的439份血清样本,并通过高效液相色谱法(HPLC)和N-Latex免疫比浊法进行分析。建立了新的特定于三个月的参考间隔。
结果:这项研究表明,CDT百分比呈三个月依赖性增加,多达39.4%的人口超过了先前确定的1.7%的参考上限。在我们的研究中,%DST/%CDT的估计参考上限为1.55%,1.96%,2.05%和1.35%的三个月1、2、3和产后的HPLC方法和2.02%,2.19%,N-Latex免疫测定为2.19%和1.96%。
结论:我们证明了妊娠期间CDT水平升高。增加的幅度取决于方法,需要考虑。我们已经建立了方法和特定于三个月的参考间隔,以防止怀孕期间酒精滥用筛查测试中的假阳性结果。