Abstract：
Bioaugmentation technology for improving the performance of thermophilic anaerobic digestion (TAD) of food waste (FW) treatment is gaining more attention. In this study, four thermophilic strains (Ureibacillus suwonensis E11, Clostridium thermopalmarium HK1, Bacillus thermoamylovorans Y25 and Caldibacillus thermoamylovorans QK5) were inoculated in the TAD of FW system, and the biochemical methane potential (BMP) batch study was conducted to assess the potential of different bioaugmented strains to enhance methane production. The results showed that the cumulative methane production in groups inoculated with E11, HK1, Y25 and QK5 improved by 2.05%, 14.54%, 19.79% and 9.17%, respectively, compared with the control group with no inoculation. Moreover, microbial community composition analysis indicated that the relative abundance of the main hydrolytic bacteria and/or methanogenic archaea was increased after bioaugmentation, and the four strains successfully became representative bacterial biomarkers in each group. The four strains enhanced methane production by strengthening starch, sucrose, galactose, pyruvate and methane metabolism functions. Further, the correlation networks demonstrated that the representative bacterial genera had positive correlations with the differential metabolic functions in each bioaugmentation group. This study provides new insights into the TAD of FW with bioaugmented strains.
摘要：
提高餐厨垃圾高温厌氧消化（TAD）处理性能的生物强化技术越来越受到重视。在这项研究中,在FW系统的TAD中接种了4株嗜热菌株（猪源UreibacillussuwonensisE11，热杆状梭菌HK1，热淀粉芽孢杆菌Y25和热淀粉芽孢杆菌QK5），并进行了生化甲烷潜力（BMP）批次研究，以评估不同生物增强菌株增强甲烷产量的潜力。结果表明,接种E11、HK1、Y25和QK5组的累积产甲烷量提高了2.05%,14.54%,19.79%和9.17%，分别,与未接种的对照组相比。此外，微生物群落组成分析表明，生物强化后主要水解菌和/或产甲烷古菌的相对丰度增加，4株菌株成功成为各组的代表性细菌生物标志物。这四个菌株通过强化淀粉来提高甲烷产量，蔗糖,半乳糖,丙酮酸和甲烷代谢功能。Further,相关网络表明，在每个生物强化组中，代表性细菌属与不同的代谢功能呈正相关。这项研究为使用生物增强菌株对FW的TAD提供了新的见解。
