Abstract：
Limited application in protecting lung health is attributed to the low levels of active compounds in lily plant bulbs. This study focused on enhancing the active compounds by fermenting Lilium davidii (Lanzhou Lily) bulbs with Limosilactobacillus fermentum GR-3, isolated from Jiangshui. Lily fermented bulbs with strain GR-3 (LFB+GR-3) increased the bioavailability of hexadecanoic acid methyl ester, 22-tetrahydroxy-5alpha-cholestan-6-one-3-O-beta-d-allopyranoside, 22-O-(6-deoxy-Alpha-l-mannopyranosyl)-3-O-beta-d-glucopyranosyl-pregn-5-en-20-one, 1-O-trans-feruloylglycerol, and 3,4 dihydroxybenzoic acid. LFB+GR-3 fraction was employed to treat the mice model exposed to the carbon black nanoparticles (CBNPs). Immunohistochemical analysis revealed that the deposition of CBNPs and damages in lung tissues were limited in the LFB+GR-3 treatment group, while TNF-α, IL-10, and IL-6 were elevated by 6.9, 4.3, and 7 folds in the CBNP exposure group. In addition, Lactobacillus, Escherichia, Lactococcus, and Muribacter were dominant in the lung microbiota of LFB+GR-3 than the CBNP group. The use of probiotic fermented lily bulbs might be helpful in lung infection treatment.
摘要：
在保护肺部健康方面的有限应用归因于百合植物鳞茎中活性化合物含量低。这项研究的重点是通过用从江水分离的发酵LimosilactobacillusGR-3发酵百合（兰州百合）鳞茎来增强活性化合物。用菌株GR-3（LFBGR-3）发酵的百合球茎增加了十六烷酸甲酯的生物利用度，22-四羟基-5α-胆甾烷-6-酮-3-O-β-d-别吡喃糖苷，22-O-（6-脱氧-α-1-吡喃甘露糖基）-3-O-β-d-吡喃葡萄糖基-pregn-5-en-20-酮，1-O-反式-阿魏酸甘油，和3,4-二羟基苯甲酸。采用LFB+GR-3级分处理暴露于炭黑纳米颗粒(CBNP)的小鼠模型。免疫组织化学分析显示,LFB+GR-3治疗组CBNPs沉积和肺组织损伤受限,而TNF-α,IL-10和IL-6在CBNP暴露组中分别升高6.9、4.3和7倍。此外,乳酸菌,埃希氏菌,乳球菌,与CBNP组相比，LFBGR-3的肺部微生物群中，Muribacter占优势。益生菌发酵百合鳞茎的使用可能有助于肺部感染的治疗。
