Abstract：
Environmental monitoring of pesticide residues in crops is essential for both food safety and environmental protection. Traditional methodologies face challenges due to the interference of endogenous compounds in peel and pulp tissues, often being invasive, labor-intensive, and inadequate for real-time observation of hazardous substance distribution. In this study, dynamic borohydride-reduced nanoparticles were employed as enhanced substrates. For the first time, surface-enhanced Raman spectroscopy (SERS) imaging was harnessed to enable whole-process visual detection of pesticide residues. The developed method is both stable and sensitive, boasting a detection lower limit below 1 pg/mL, coupled with robust quantitative analytical capabilities. This technique was successfully employed to detect residue signals across various crops and fruit juices. Furthermore, SERS imaging was utilized to map the distribution of pesticide residues from the exterior to the interior of fruits and vegetables. Vertex component analysis further refined the process by mitigating interference from plant autofluorescence. Collectively, this innovative strategy facilitates comprehensive pesticide residue monitoring, offering a potent tool for controlling hazardous substances in crops. Its potential applications extend beyond food safety, holding significant promise for sustainable agricultural production and enhanced environmental safeguarding.
摘要：
农作物农药残留的环境监测对于食品安全和环境保护都至关重要。传统的方法面临的挑战，由于在果皮和牙髓组织内源性化合物的干扰，通常是侵入性的，劳动密集型,不足以实时观察有害物质的分布。在这项研究中,动态硼氢化物还原的纳米粒子被用作增强的底物。第一次,利用表面增强拉曼光谱（SERS）成像来实现农药残留的全过程视觉检测。所开发的方法既稳定又灵敏，检测下限低于1pg/mL，再加上强大的定量分析能力。该技术已成功用于检测各种作物和果汁中的残留信号。此外，SERS成像用于绘制从水果和蔬菜外部到内部的农药残留分布图。顶点成分分析通过减轻植物自发荧光的干扰进一步完善了该过程。总的来说，这一创新战略促进了全面的农药残留监测，为控制农作物中的有害物质提供了有力的工具。它的潜在应用范围超出了食品安全，对可持续农业生产和加强环境保护抱有重大希望。
