PDF(Pubmed)
Abstract：
Exogenous nutrients are essential for body and skeletal muscle growth in newly hatched chicks, and delaying post-hatch feeding negatively affects body growth, meat yield, and meat quality. The aim of this study was to investigate the effects of delayed post-hatch feeding on the metabolic profiles of broiler chickens using a combination of targeted and untargeted metabolomics. Newly hatched chicks had either immediate free access to feed (freely fed chicks) or no access to feed from 0 to 2 days of age (delayed-fed chicks); both groups were subsequently provided feed ad libitum until 13 days of age. Untargeted metabolomic analysis was performed using gas chromatography-mass spectrometry, whereas targeted metabolomic analysis of amino acids was performed using high-performance liquid chromatography with ortho-phthalaldehyde derivatization. Delayed feeding increased the plasma levels of sucrose, maltose, serotonin, lactitol, gentiobiose, xylitol, threonic acid, and asparagine, and decreased the plasma levels of creatinine, aspartic acid, and glutamic acid. In addition, the digestibility of the nitrogen-free extract (starch and sugar) and the cecal butyric acid concentration increased in chicks subjected to delayed feeding. In contrast, delayed feeding did not affect muscle protein degradation or digestibility in chicks. Taken together, our results indicate that delaying feeding until 48 h post-hatch alters multiple metabolic pathways, which are accompanied by changes in intestinal carbohydrate digestion and cecal butyric acid content in broiler chickens.
摘要：
在刚孵化的小鸡中，外源性营养素对于身体和骨骼肌的生长是必不可少的，延迟孵化后的进食会对身体生长产生负面影响，肉类产量，和肉的质量。本研究的目的是使用靶向和非靶向代谢组学的组合研究孵化后延迟喂养对肉鸡代谢谱的影响。新孵化的小鸡可以立即自由获取饲料（自由饲喂的小鸡），或者从0至2日龄无法获取饲料（延迟饲喂的小鸡）；随后，两组均免费提供饲料，直至13日龄。使用气相色谱-质谱法进行非目标代谢组学分析，而氨基酸的靶向代谢组学分析是使用高效液相色谱法与邻苯二甲醛衍生化进行的.延迟喂食增加了蔗糖的血浆水平，麦芽糖,血清素,乳糖醇，龙胆二糖,木糖醇,苏糖酸，和天冬酰胺,并降低了血浆肌酐水平，天冬氨酸,还有谷氨酸.此外,在延迟饲喂的雏鸡中，无氮提取物（淀粉和糖）的消化率和盲肠丁酸浓度增加。相比之下,延迟饲喂不会影响雏鸡的肌肉蛋白质降解或消化率。一起来看,我们的结果表明，延迟喂食至孵化后48小时会改变多种代谢途径，伴随着肉鸡肠道碳水化合物消化和盲肠丁酸含量的变化。
