PDF(Pubmed)
Abstract：
The simultaneous determination of multi-mycotoxins in food commodities are highly desirable due to their potential toxic effects and mass consumption of foods. Herein, liquid chromatography-quadrupole exactive orbitrap mass spectrometry was proposed to analyze multi-mycotoxins in commercial vegetable oils. Specifically, the method featured a successive liquid-liquid extraction process, in which the complementary solvents consisted of acetonitrile and water were optimized. Resultantly, matrix effects were reduced greatly. External calibration approach revealed good quantification property for each analyte. Under optimal conditions, the recovery ranging from 80.8% to 109.7%, relative standard deviation less than 11.7%, and good limit of quantification (0.35 to 45.4 ng/g) were achieved. The high accuracy of proposed method was also validated. The detection of 20 commercial vegetable oils revealed that aflatoxins B1 and B2, zearalenone were observed in 10 real samples. The as-developed method is simple and low-cost, which merits the wide applications for scanning mycotoxins in oil matrices.
摘要：
由于食品中的多种真菌毒素的潜在毒性作用和大量食用，因此非常需要同时测定食品中的多种真菌毒素。在这里，提出了液相色谱-四极杆反应性轨道阱质谱分析商品植物油中多种真菌毒素的方法。具体来说,该方法具有连续的液-液萃取过程，其中对乙腈和水组成的补充溶剂进行了优化。结果，基体效应大大降低。外部校准方法显示出每种分析物的良好定量特性。在最优条件下,回收率从80.8%到109.7%，相对标准偏差小于11.7%，并达到了良好的定量限（0.35至45.4ng/g）。验证了该方法的高精度。对20种商品植物油的检测表明，在10种实际样品中观察到黄曲霉毒素B1和B2，玉米赤霉烯酮。所开发的方法简单、成本低,值得在油基质中扫描真菌毒素的广泛应用。
