Abstract：
Golden tide outbreak threatened the marine ecological environment. Sargassum horneri is a single dominant species of the Yellow Sea golden tide, which growth and development are affected by changes in sea water temperature. This study investigated the photosynthetic physiology of copper algae and found that the growth rate, chlorophyll a content, carotenoid content, Fv/Fm, and maximum electron transfer efficiency were significantly reduced, indicating that Sargassum horneri was under stress under high temperature. In this study, high-throughput sequencing was used to analyze the response mechanisms of photosynthesis-related genes in S. horneri under high temperature stress. The results showed that most of the photosynthesis-related genes in S. horneri were downregulated and photosynthesis was inhibited under high temperature stress. However, the expression levels of ferredoxin, ferredoxin-NADP reductase, light-harvesting protein complexes, and oxygen-evolving complex genes were significantly upregulated (P ≤ 0.05) after five days of high temperature treatment. This study found that photosynthesis related genes play a crucial role in regulating the photosynthetic response of S. horneri to high temperature stress.
摘要：
金潮爆发威胁着海洋生态环境。Sargassumhorneri是黄海金潮的单一优势种，其生长发育受海水温度变化的影响。本研究调查了铜藻的光合生理,叶绿素a含量,类胡萝卜素含量，Fv/Fm,最大电子转移效率显著降低,表明马尾藻在高温下处于胁迫状态。在这项研究中,采用高通量测序技术分析了霍氏芽孢杆菌光合作用相关基因在高温胁迫下的响应机制。结果表明,在高温胁迫下,刺梨光合作用相关基因大部分下调,光合作用受到抑制。然而,铁氧还蛋白的表达水平，铁氧还蛋白-NADP还原酶，捕光蛋白质复合物，高温处理五天后，氧释放复杂基因显着上调（P≤0.05）。本研究发现,光合作用相关基因在调节霍氏芽孢杆菌对高温胁迫的光合响应中起着至关重要的作用。
