PDF(Pubmed)
Abstract：
BACKGROUND: Diabetes mellitus (DM) is a non-communicable, life-threatening syndrome that is present all over the world. The use of eco-friendly, cost-effective, and green-synthesised nanoparticles as a medicinal therapy in the treatment of DM is an attractive option.
OBJECTIVE: In the present study, silver nanoparticles (AI-AgNPs) were biosynthesized through the green synthesis method using Azadirachta indica seed extract to evaluate their anti-diabetic potentials.
METHODS: These nanoparticles were characterized by using UV-visible spectroscopy, Fourier transform infrared spectrophotometers (FTIR), scanning electron microscopy (SEM), DLS, and X-ray diffraction (XRD). The biosynthesized AI-AgNPs and crude extracts of Azadirachta indica seeds were evaluated for anti-diabetic potentials using glucose adsorption assays, glucose uptake by yeast cells assays, and alpha-amylase inhibitory assays.
RESULTS: Al-AgNPs showed the highest activity (75 ± 1.528%), while crude extract showed (63 ± 2.5%) glucose uptake by yeast at 80 µg/mL. In the glucose adsorption assay, the highest activity of Al-AgNPs was 10.65 ± 1.58%, while crude extract showed 8.32 ± 0.258% at 30 mM, whereas in the alpha-amylase assay, Al-AgNPs exhibited the maximum activity of 73.85 ± 1.114% and crude extract 65.85 ± 2.101% at 100 µg/mL. The assay results of AI-AgNPs and crude showed substantial dose-dependent activities. Further, anti-diabetic potentials were also investigated in streptozotocin-induced diabetic mice. Mice were administered with AI-AgNPs (10 to 40 mg/kg b.w) for 30 days.
CONCLUSIONS: The results showed a considerable drop in blood sugar levels, including pancreatic and liver cell regeneration, demonstrating that AI-AgNPs have strong anti-diabetic potential.
OBJECTIVE: In the present study, silver nanoparticles (AI-AgNPs) were biosynthesized through the green synthesis method using Azadirachta indica seed extract to evaluate their anti-diabetic potentials.
METHODS: These nanoparticles were characterized by using UV-visible spectroscopy, Fourier transform infrared spectrophotometers (FTIR), scanning electron microscopy (SEM), DLS, and X-ray diffraction (XRD). The biosynthesized AI-AgNPs and crude extracts of Azadirachta indica seeds were evaluated for anti-diabetic potentials using glucose adsorption assays, glucose uptake by yeast cells assays, and alpha-amylase inhibitory assays.
RESULTS: Al-AgNPs showed the highest activity (75 ± 1.528%), while crude extract showed (63 ± 2.5%) glucose uptake by yeast at 80 µg/mL. In the glucose adsorption assay, the highest activity of Al-AgNPs was 10.65 ± 1.58%, while crude extract showed 8.32 ± 0.258% at 30 mM, whereas in the alpha-amylase assay, Al-AgNPs exhibited the maximum activity of 73.85 ± 1.114% and crude extract 65.85 ± 2.101% at 100 µg/mL. The assay results of AI-AgNPs and crude showed substantial dose-dependent activities. Further, anti-diabetic potentials were also investigated in streptozotocin-induced diabetic mice. Mice were administered with AI-AgNPs (10 to 40 mg/kg b.w) for 30 days.
CONCLUSIONS: The results showed a considerable drop in blood sugar levels, including pancreatic and liver cell regeneration, demonstrating that AI-AgNPs have strong anti-diabetic potential.
摘要：
背景：糖尿病（DM）是一种非传染性疾病，威胁生命的综合征，存在于世界各地。使用环保，成本效益高,和绿色合成的纳米颗粒作为治疗DM的药物疗法是一个有吸引力的选择。
目的：在本研究中，银纳米颗粒（AI-AgNPs）是通过绿色合成方法使用印版种子提取物生物合成的，以评估其抗糖尿病潜力。
方法：使用紫外可见光谱对这些纳米粒子进行表征，傅里叶变换红外分光光度计(FTIR),扫描电子显微镜（SEM），DLS,和X射线衍射(XRD)。使用葡萄糖吸附测定法评估了生物合成的AI-AgNPs和印染种子的粗提物的抗糖尿病潜力，酵母细胞的葡萄糖摄取分析，和α-淀粉酶抑制试验。
结果：Al-AgNPs显示出最高的活性（75±1.528％），而粗提物显示（63±2.5％）葡萄糖被酵母吸收为80µg/mL。在葡萄糖吸附试验中,Al-AgNPs的最高活性为10.65±1.58%，而粗提物在30mM时显示8.32±0.258％，而在α-淀粉酶检测中，在100µg/mL时，Al-AgNP的最大活性为73.85±1.114％，粗提取物为65.85±2.101％。AI-AgNP和粗品的测定结果显示出显著的剂量依赖性活性。Further,还在链脲佐菌素诱导的糖尿病小鼠中研究了抗糖尿病潜力。给小鼠施用AI-AgNP(10至40mg/kgb.w)30天。
结论：结果显示血糖水平大幅下降，包括胰腺和肝细胞再生，证明AI-AgNP具有很强的抗糖尿病潜力。
目的：在本研究中，银纳米颗粒（AI-AgNPs）是通过绿色合成方法使用印版种子提取物生物合成的，以评估其抗糖尿病潜力。
方法：使用紫外可见光谱对这些纳米粒子进行表征，傅里叶变换红外分光光度计(FTIR),扫描电子显微镜（SEM），DLS,和X射线衍射(XRD)。使用葡萄糖吸附测定法评估了生物合成的AI-AgNPs和印染种子的粗提物的抗糖尿病潜力，酵母细胞的葡萄糖摄取分析，和α-淀粉酶抑制试验。
结果：Al-AgNPs显示出最高的活性（75±1.528％），而粗提物显示（63±2.5％）葡萄糖被酵母吸收为80µg/mL。在葡萄糖吸附试验中,Al-AgNPs的最高活性为10.65±1.58%，而粗提物在30mM时显示8.32±0.258％，而在α-淀粉酶检测中，在100µg/mL时，Al-AgNP的最大活性为73.85±1.114％，粗提取物为65.85±2.101％。AI-AgNP和粗品的测定结果显示出显著的剂量依赖性活性。Further,还在链脲佐菌素诱导的糖尿病小鼠中研究了抗糖尿病潜力。给小鼠施用AI-AgNP(10至40mg/kgb.w)30天。
结论：结果显示血糖水平大幅下降，包括胰腺和肝细胞再生，证明AI-AgNP具有很强的抗糖尿病潜力。
