OBJECTIVE: In the present study, silver nanoparticles (AI-AgNPs) were biosynthesized through the green synthesis method using Azadirachta indica seed extract to evaluate their anti-diabetic potentials.
METHODS: These nanoparticles were characterized by using UV-visible spectroscopy, Fourier transform infrared spectrophotometers (FTIR), scanning electron microscopy (SEM), DLS, and X-ray diffraction (XRD). The biosynthesized AI-AgNPs and crude extracts of Azadirachta indica seeds were evaluated for anti-diabetic potentials using glucose adsorption assays, glucose uptake by yeast cells assays, and alpha-amylase inhibitory assays.
RESULTS: Al-AgNPs showed the highest activity (75 ± 1.528%), while crude extract showed (63 ± 2.5%) glucose uptake by yeast at 80 µg/mL. In the glucose adsorption assay, the highest activity of Al-AgNPs was 10.65 ± 1.58%, while crude extract showed 8.32 ± 0.258% at 30 mM, whereas in the alpha-amylase assay, Al-AgNPs exhibited the maximum activity of 73.85 ± 1.114% and crude extract 65.85 ± 2.101% at 100 µg/mL. The assay results of AI-AgNPs and crude showed substantial dose-dependent activities. Further, anti-diabetic potentials were also investigated in streptozotocin-induced diabetic mice. Mice were administered with AI-AgNPs (10 to 40 mg/kg b.w) for 30 days.
CONCLUSIONS: The results showed a considerable drop in blood sugar levels, including pancreatic and liver cell regeneration, demonstrating that AI-AgNPs have strong anti-diabetic potential.
目的:在本研究中,银纳米颗粒(AI-AgNPs)是通过绿色合成方法使用印版种子提取物生物合成的,以评估其抗糖尿病潜力。
方法:使用紫外可见光谱对这些纳米粒子进行表征,傅里叶变换红外分光光度计(FTIR),扫描电子显微镜(SEM),DLS,和X射线衍射(XRD)。使用葡萄糖吸附测定法评估了生物合成的AI-AgNPs和印染种子的粗提物的抗糖尿病潜力,酵母细胞的葡萄糖摄取分析,和α-淀粉酶抑制试验。
结果:Al-AgNPs显示出最高的活性(75±1.528%),而粗提物显示(63±2.5%)葡萄糖被酵母吸收为80µg/mL。在葡萄糖吸附试验中,Al-AgNPs的最高活性为10.65±1.58%,而粗提物在30mM时显示8.32±0.258%,而在α-淀粉酶检测中,在100µg/mL时,Al-AgNP的最大活性为73.85±1.114%,粗提取物为65.85±2.101%。AI-AgNP和粗品的测定结果显示出显著的剂量依赖性活性。Further,还在链脲佐菌素诱导的糖尿病小鼠中研究了抗糖尿病潜力。给小鼠施用AI-AgNP(10至40mg/kgb.w)30天。
结论:结果显示血糖水平大幅下降,包括胰腺和肝细胞再生,证明AI-AgNP具有很强的抗糖尿病潜力。