PDF(Pubmed)
Abstract：
Organizations like the Clinical and Laboratory Standards Institute (CLSI) or the European Committee of Antimicrobial Susceptibility Testing (EUCAST) provide standardized methodologies for antimicrobial susceptibility testing of a wide range of nonfastidious and fastidious bacteria, but so far not for Mycoplasma spp. of animal origin. Recently, a proposed method for the standardized broth microdilution testing of Mycoplasma hyorhinis using commercial Sensititre microtiter plates was presented. In this study, we evaluated this broth microdilution method with 37 field isolates and tested their susceptibility toward the following antimicrobial agents: doxycycline, enrofloxacin, erythromycin, florfenicol, gentamicin, marbofloxacin, tetracycline, tiamulin, tilmicosin, tulathromycin, and tylosin. The isolates originated from different countries, isolation sites, and years. The broth microdilution method was carried out using a modified Friis broth as the culture and test medium. For macrolides and lincosamides, a bimodal distribution with elevated MIC values could be observed for almost half of the tested field isolates, deducing reduced susceptibility toward these substances. With a recently published protocol, we were able to test a variety of field isolates, and consistent data could be obtained. Using this method, monitoring studies of Mycoplasma hyorhinis isolates can be carried out in a comparable manner, and the observed susceptibility profiles can be screened for possible changes in MIC values in the future.
摘要：
临床和实验室标准研究所（CLSI）或欧洲抗菌药物敏感性测试委员会（EUCAST）等组织为各种非挑剔和挑剔的细菌提供了抗菌药物敏感性测试的标准化方法。但到目前为止还没有支原体。动物起源。最近,提出了一种使用商业Sensittre微量滴定板对猪鼻支原体进行标准化肉汤微量稀释测试的方法。在这项研究中,我们用37种现场分离物评估了这种肉汤微量稀释方法，并测试了它们对以下抗菌剂的敏感性：多西环素，恩诺沙星,红霉素,氟苯尼考,庆大霉素,马波沙星,四环素,tiamulin,替米考星,泰拉霉素,还有泰乐菌素.这些分离株来自不同的国家，隔离站点,和几年。使用改良的Friis肉汤作为培养物和测试培养基进行肉汤微量稀释方法。对于大环内酯类和lincosamides，几乎一半的测试现场分离株可以观察到MIC值升高的双峰分布，推断对这些物质的敏感性降低。根据最近发布的协议，我们能够测试各种野外分离株，可以获得一致的数据。使用此方法，猪鼻支原体分离株的监测研究可以以类似的方式进行，并且可以筛选观察到的敏感性曲线，以了解将来MIC值的可能变化。
