PDF(Pubmed)
Abstract：
Methicillin-resistant Staphylococcus aureus (MRSA) colonization has been considered a risk factor for the development of infection, however, there are no studies that have compared the colonizing and infecting strains using whole-genome sequencing (WGS). The aim of this study is to determine the prevalence of and risk factors for MRSA colonization among long-term care facilities (LTCF) residents of Tenerife (Spain), and to analyze the epidemiological relationship between the colonizing and infecting strains using WGS. A point-prevalence study was carried out at 14 LTCFs in Tenerife from October 2020 to May 2021. Nasal swabs were cultured for MRSA. Colonized residents were followed up for two years. A phylogenetic comparison between colonization and infection strains was performed using WGS. A total of 764 residents were included. The prevalence of colonization by MRSA was 28.1% (n = 215), of which 12 (5.6%) subsequently developed infection. A close genetic relationship between colonization and infection isolates was found in three of the four (75%) residents studied. Our study confirms that colonized residents can develop serious MRSA infections from the same nasal colonization strain. Given the high prevalence of MRSA colonization in these centers, it is necessary to implement strategies with preventive measures to avoid the development of infection and the transmission of MRSA.
摘要：
耐甲氧西林金黄色葡萄球菌（MRSA）定植被认为是感染发展的危险因素。然而,没有研究使用全基因组测序(WGS)比较定殖和感染菌株.这项研究的目的是确定特内里费岛（西班牙）长期护理设施（LTCF）居民中MRSA定植的患病率和危险因素，并利用WGS分析定殖和感染菌株之间的流行病学关系。2020年10月至2021年5月，在特内里费岛的14个LTCF进行了点患病率研究。针对MRSA培养鼻拭子。殖民地居民被跟踪了两年。使用WGS进行定殖和感染菌株之间的系统发育比较。总共包括764名居民。MRSA定植的患病率为28.1%(n=215),其中12人(5.6%)随后出现感染。在所研究的四名居民中的三名（75％）中发现了定植与感染分离株之间的紧密遗传关系。我们的研究证实，定植的居民可以从相同的鼻定植菌株中发展出严重的MRSA感染。鉴于这些中心MRSA定植的高患病率，有必要实施具有预防措施的策略，以避免感染的发展和MRSA的传播。
