PDF(Pubmed)
Abstract：
Sea buckthorn (Hippophae rhamnoides L.) (SB) is increasingly consumed worldwide as a food and food supplement. The remarkable richness in biologically active phytochemicals (polyphenols, carotenoids, sterols, vitamins) is responsible for its purported nutritional and health-promoting effects. Despite the considerable interest and high market demand for SB-based supplements, a limited number of studies report on the authentication of such commercially available products. Herein, untargeted metabolomics based on ultra-high-performance liquid chromatography coupled with quadrupole-time of flight mass spectrometry (UHPLC-QTOF-ESI+MS) were able to compare the phytochemical fingerprint of leaves, berries, and various categories of SB-berry herbal supplements (teas, capsules, tablets, liquids). By untargeted metabolomics, a multivariate discrimination analysis and a univariate approach (t-test and ANOVA) showed some putative authentication biomarkers for berries, e.g., xylitol, violaxanthin, tryptophan, quinic acid, quercetin-3-rutinoside. Significant dominant molecules were found for leaves: luteolin-5-glucoside, arginine, isorhamnetin 3-rutinoside, serotonin, and tocopherol. The univariate analysis showed discriminations between the different classes of food supplements using similar algorithms. Finally, eight molecules were selected and considered significant putative authentication biomarkers. Further studies will be focused on quantitative evaluation.
摘要：
沙棘(HippophaerhamnoidesL.)(SB)作为食品和食品补充剂在世界范围内越来越多地消费。生物活性植物化学物质(多酚，类胡萝卜素，固醇,维生素）负责其所谓的营养和健康促进作用。尽管对基于SB的补充剂有相当大的兴趣和很高的市场需求，关于此类市售产品认证的数量有限的研究报告。在这里，基于超高效液相色谱-四极杆-飞行时间质谱(UHPLC-QTOF-ESI+MS)的非靶向代谢组学能够比较叶片的植物化学指纹图谱,浆果,和各种类别的SB-浆果草药补充剂(茶，胶囊,片剂,液体)。通过非靶向代谢组学，多变量判别分析和单变量方法（t检验和方差分析）显示了浆果的一些推定认证生物标志物，例如，木糖醇,紫黄质,色氨酸,奎尼酸，槲皮素-3-rutinoside。在叶子中发现了重要的显性分子：木犀草素-5-葡萄糖苷，精氨酸异鼠李素3-鲁特诺苷，血清素,和生育酚.单变量分析显示了使用类似算法的不同类别的食品补充剂之间的区别。最后,选择了8个分子,并将其视为有意义的推定认证生物标志物.进一步的研究将集中在定量评估上。
