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Abstract：
China Xihu Longjing tea is famous for its good flavor and quality. However, information on its related metabolites, except for flavonoids, is largely deficient. Different processing methods for China Xihu Longjing tea fixing-by machines at both the first and second step (A1), first step by machine and second step by hand (A2), first step by hand and second step by machine (A3), and by hand at both the first and second step (A4)-were compared using a UHPLC-QE-MS-based metabolomics approach. Liquid chromatography-mass spectrometry was used to analyze the metabolic profiles of the processed samples. A total of 490 metabolites (3 alkaloids, 3 anthracenes, 15 benzene and substituted derivatives, 2 benzopyrans, 13 coumarins and derivatives, 128 flavonoids, 4 furanoid lignans, 16 glycosides and derivatives, 5 indoles and derivatives, 18 isocoumarins and derivatives, 4 chalcones and dihydrochalcones, 4 naphthopyrans, 3 nucleosides, 78 organic acids and derivatives, 55 organooxygen compounds, 5 phenols, 109 prenol lipids, 3 saccharolipids, 3 steroids and steroid derivatives, and 17 tannins) were identified. The different metabolic profiles were distinguished using PCA and OPLS-DA. There were differences in the types and contents of the metabolites, especially flavonoids, furanoid lignans, glycosides and derivatives, organic acids and derivatives, and organooxygen compounds. There was a positive correlation between flavonoid metabolism and amino acid metabolism. However, there was a negative correlation between flavonoid metabolism and amino acid metabolism, which had the same trend as prenol lipid metabolism and tannins. This study provides new valuable information regarding differences in the metabolite profile of China Xihu Longjing tea processed based on machine fixing and on manual fixing methods.
摘要：
中国西湖龙井茶以其良好的风味和质量而闻名。然而,有关其相关代谢物的信息，除了类黄酮,很大程度上是有缺陷的。中国西湖龙井茶固定机的不同加工方法在第一和第二步（A1），第一步是机器，第二步是手工（A2），第一步手工，第二步机器（A3），使用基于UHPLC-QE-MS的代谢组学方法对第一步和第二步（A4）进行了比较。使用液相色谱-质谱法分析处理过的样品的代谢谱。共有490种代谢物(3种生物碱，3蒽，15苯和取代的衍生物，2苯并吡喃，13香豆素及其衍生物，128类黄酮，4呋喃类木脂素，16糖苷及其衍生物，5吲哚及其衍生物，18异香豆素及其衍生物，4个查耳酮和二氢查耳酮，4个萘并吡喃，3个核苷，78有机酸及其衍生物，55种有机氧化合物，5酚，109prenol脂质，3糖脂，3类固醇和类固醇衍生物，和17种单宁)被鉴定。使用PCA和OPLS-DA区分不同的代谢谱。代谢物的种类和含量存在差异,尤其是类黄酮,呋喃类木脂素，糖苷和衍生物，有机酸及其衍生物，和有机氧化合物。黄酮代谢与氨基酸代谢呈正相关。然而,黄酮代谢与氨基酸代谢呈负相关,与普瑞诺脂质代谢和单宁具有相同的趋势。这项研究提供了有关基于机器固定和手动固定方法加工的中国西湖龙井茶的代谢谱差异的新的有价值的信息。
