PDF(Pubmed)
Abstract：
A longitudinal design with a simple random sampling method was used to collect and compare microbial hygiene levels between the dry season (January to April) and wet season (June to August). A total of 456 milk and cottage cheese samples were collected from each site along the dairy value chain from three regions. Enumeration of total aerobic mesophilic bacteria (APC), total coliforms (TCC), and Escherichia coli (EC) was performed according to standard methods. Independent t-tests were employed to assess the significant variation at (p < 0.05) between the two seasons. The cumulative result of APC of 7.61 log cfu/mL and g and TCC of 3.50 log cfu/mL in the dry season were significantly higher than the wet season of 7.15 log cfu/mL and 2.49 log cfu/mL, respectively, whereas generic E. coli count (EC) was significantly higher in the wet season (0.70 log cfu/mL and g) than that in the dry season (0.40 log cfu/mL and g). The results of hygienic indicator microbial load significantly varied with season. Hence, hygienic milk production and handling practices that comprehend seasonal influence should be implemented to improve the safety of milk.
摘要：
采用简单随机抽样方法的纵向设计用于收集和比较旱季（1月至4月）和雨季（6月至8月）之间的微生物卫生水平。从三个地区的乳制品价值链中的每个站点收集了总共456个牛奶和奶酪样品。总需氧嗜温细菌（APC）的计数，总大肠菌群(TCC)，和大肠杆菌(EC)根据标准方法进行。采用独立的t检验来评估两个季节之间在(p<0.05)处的显著变化。旱季的APC为7.61logcfu/mL和g，TCC为3.50logcfu/mL的累积结果明显高于雨季的7.15logcfu/mL和2.49logcfu/mL，分别,而普通大肠杆菌计数（EC）在雨季（0.70logcfu/mL和g）明显高于旱季（0.40logcfu/mL和g）。卫生指标微生物负荷的结果随季节显著变化。因此，应实施了解季节性影响的卫生牛奶生产和处理措施，以提高牛奶的安全性。
