Abstract：
This study presents a genome-wide identification of NOD-like receptors (NLRs) in the golden pompano, key to its innate immunity. We identified 30 ToNLRs, analyzing their chromosomal positions, characteristics, evolutionary relationships, evidence of positive selection, and synteny with the yellowtail kingfish. Our findings categorize these NLRs into three main subgroups: NLRA, NLRC, and the distinct ToNLRX1. Post-exposure to Streptococcus agalactiae, most ToNLRs increased expression in the spleen, whereas NLRC3like13, NLRC3like16, and NLRC3like19 so in the kidneys. Upon Cryptocaryon irritans exposure, we categorized our groups based on the site of infection into the control group (BFS), the trophont-attached skin (TAS), and the nearby region skin (NRS). ToAPAF1 and ToNOD1 expressions rose in the NRS, in contrast to decreased expressions of ToNLRC5, ToNWD1 and ToCIITA. Other ToNLRs showed variable expressions in the TAS. Overall, this research lays the groundwork for further exploration of innate immunity in the golden pompano.
摘要：
这项研究提出了一种全基因组鉴定的NOD样受体（NLRs）在金pompano，它先天免疫的关键。我们确定了30个ToNLR,分析它们的染色体位置，特点,进化关系，积极选择的证据，与黄尾王鱼合音。我们的发现将这些NLR分为三个主要亚组：NLRA，NLRC,和独特的ToNLRX1。接触无乳链球菌后，大多数ToNLR在脾脏中的表达增加，而NLRC3like13，NLRC3like16和NLRC3like19在肾脏中如此。隐虫暴徒暴露后，我们根据感染部位将我们的组分为对照组（BFS），附着有托的皮肤（TAS），和附近区域皮肤(NRS)。ToAPAF1和ToNOD1表达在NRS中上升，与ToNLRC5、ToNWD1和ToCIITA的表达降低相反。其他ToNLR在TAS中显示可变表达。总的来说,本研究为进一步探索金帕诺先天免疫奠定了基础。
