Abstract：
CONCLUSIONS: Sweet cherry PavbHLH106 was up-regulated under cold induction and overexpressed to enhance the cold resistance in tobacco by mediating the scavenging of ROS through increasing of antioxidant enzyme activity. Sweet cherry (Prunus avium L.) is an economically important fruit. Chilling requirements are critical during dormancy, but abnormally low temperatures unfavorably affect fruit growth and development. Differences were found in the transcript level of PavbHLH106 under salt, dehydration, and low-temperature treatments, especially in response to cold stress, suggesting that this gene is involved in the regulation of different abiotic stresses. PavbHLH106 is homologous to Arabidopsis thaliana AtbHLH106 with a conserved bHLH domain, and transient expression in tobacco suggests that the protein is localized in the nucleus and has transcriptional activity in yeast. The PavbHLH106 overexpression in tobacco resulted in weaker electrolyte leakages, lower malondialdehyde, and higher proline content than the wild type at low-temperature treatment. Reactive oxygen species accumulation was significantly reduced in the overexpressed lines, negatively correlated with the antioxidant enzyme activity. In addition, overexpression of PavbHLH106 delayed the germination of tobacco seeds and promoted plant growth. Resistance-related genes were expressed more in the overexpressed plants compared to the wild type. PavbHLH106 bound to the PavACO promoter in yeast and potentially interacted with a bHLH162-like transcription factor. These results indicate that PavbHLH106 has various functions and is particularly active in controlling low-temperature stress.
摘要：
结论：甜樱桃PavbHLH106在低温诱导下表达上调，并通过增加抗氧化酶活性介导清除ROS来增强烟草的抗寒性。甜樱桃（PrunusaviumL.）是一种重要的经济水果。在休眠期间，冷却要求至关重要，但异常低温不利于果实的生长发育。在盐下PavbHLH106的转录水平存在差异，脱水,和低温处理，尤其是对寒冷压力的反应，这表明该基因参与了不同非生物胁迫的调控。PavbHLH106与拟南芥AtbHLH106同源，具有保守的bHLH结构域，在烟草中的瞬时表达表明该蛋白质位于细胞核中，并在酵母中具有转录活性。烟草中的PavbHLH106过表达导致电解质泄漏较弱，降低丙二醛，在低温处理下脯氨酸含量高于野生型。在过表达的品系中，活性氧的积累显着减少，与抗氧化酶活性呈负相关。此外,过表达PavbHLH106可延缓烟草种子的萌发，促进植物生长。与野生型相比，抗性相关基因在过表达的植物中表达更多。PavbHLH106与酵母中的PavACO启动子结合，并可能与bHLH162样转录因子相互作用。这些结果表明PavbHLH106具有各种功能，并且在控制低温胁迫方面特别活跃。
