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Abstract：
Avipoxvirus (APV), a linear dsDNA virus belonging to the subfamily Chordopoxvirinae of the family Poxviridae, infects more than 278 species of domestic and wild birds. It is responsible for causing avian pox disease, characterized by its cutaneous and diphtheric forms. With a high transmission capacity, it can cause high economic losses and damage to the ecosystem. Several diagnostic methods are available, and bird vaccination can be an effective preventive measure. Ten APV-positive samples were analyzed to update the molecular characterization and phylogenetic analysis of viruses isolated in Portugal between 2017 and 2023. A P4b gene fragment was amplified using a PCR, and the nucleotide sequence of the amplicons was determined using Sanger sequencing. The sequences obtained were aligned using ClustalW, and a maximum likelihood phylogenetic tree was constructed. With this study, it was possible to verify that the analyzed sequences are distributed in subclades A1, A2, B1, and B3. Since some of them are quite similar to others from different countries and obtained in different years, it is possible to conclude that there have been several viral introductions in Portugal. Finally, it was possible to successfully update the data on Avipoxviruses in Portugal.
摘要：
禽痘病毒(APV),线性dsDNA病毒，属于Poxviridae家族的Chordopoxvirinae，感染超过278种家养和野生鸟类。它是导致禽痘疾病的原因，以其皮肤和白喉形式为特征。具有较高的传输容量，它可能会造成巨大的经济损失和对生态系统的破坏。有几种诊断方法可用，鸟类疫苗接种是一种有效的预防措施。分析了10个APV阳性样品，以更新2017年至2023年在葡萄牙分离的病毒的分子特征和系统发育分析。使用PCR扩增P4b基因片段,并且使用Sanger测序确定扩增子的核苷酸序列。使用ClustalW比对获得的序列，并构建了最大似然系统发育树。通过这项研究,可以验证所分析的序列分布在亚分化A1,A2,B1和B3中.由于其中一些与来自不同国家并在不同年份获得的其他非常相似，可以得出结论,在葡萄牙已经出现了一些病毒的引入.最后,有可能成功更新葡萄牙的禽痘病毒数据。
