PDF(Pubmed)
Abstract：
Biosensors based on an oxygen electrode, a mediator electrode, and a mediator microbial biofuel cell (MFC) using the bacteria Gluconobacter oxydans B-1280 were formed and tested to determine the integral toxicity. G. oxydans bacteria exhibited high sensitivity to the toxic effects of phenol, 2,4-dinitrophenol, salicylic and trichloroacetic acid, and a number of heavy metal ions. The system \"G. oxydans bacteria-ferrocene-graphite-paste electrode\" was superior in sensitivity to biosensors formed using an oxygen electrode and MFC, in particular regarding heavy metal ions (EC50 of Cr3+, Mn2+, and Cd2+ was 0.8 mg/dm3, 0.3 mg/dm3 and 1.6 mg/dm3, respectively). It was determined that the period of stable functioning of electrochemical systems during measurements was reduced by half (from 30 to 15 days) due to changes in the enzyme system of microbial cells when exposed to toxicants. Samples of the products made from polymeric materials were analyzed using developed biosensor systems and standard biotesting methods based on inhibiting the growth of duckweed Lemna minor, reducing the motility of bull sperm, and quenching the luminescence of the commercial test system \"Ecolum\". The developed bioelectrocatalytic systems were comparable in sensitivity to commercial biosensors, which made it possible to correlate the results and identify, by all methods, a highly toxic sample containing diphenylmethane-4,4\'-diisocyanate according to GC-MS data.
摘要：
基于氧电极的生物传感器，介体电极，和使用细菌氧化葡糖酸杆菌B-1280的介体微生物生物燃料电池（MFC）形成并测试以确定整体毒性。G.oxydans细菌对苯酚的毒性作用表现出很高的敏感性，2,4-二硝基苯酚,水杨酸和三氯乙酸，还有一些重金属离子.系统\"G.氧化物细菌-二茂铁-石墨糊电极的灵敏度优于使用氧电极和MFC形成的生物传感器，特别是关于重金属离子(Cr3+的EC50，Mn2+,和Cd2+分别为0.8mg/dm3、0.3mg/dm3和1.6mg/dm3)。已确定，由于暴露于毒物时微生物细胞的酶系统发生变化，电化学系统在测量过程中的稳定运行时间减少了一半（从30到15天）。使用开发的生物传感器系统和基于抑制浮萍小浮萍生长的标准生物测试方法分析了由聚合物材料制成的产品样品，降低公牛精子的运动性,并猝灭商业测试系统“Ecolum”的发光。开发的生物电催化系统在灵敏度上与商业生物传感器相当，这使得关联结果和识别成为可能，通过所有方法，根据GC-MS数据，含有二苯基甲烷-4,4'-二异氰酸酯的剧毒样品。
