RESULTS: The genome of isolate yu01 from a breast specimen was sequenced, and 35 CKC genomes were collected. Analysis of 16S rRNA, rpoB, and fusA suggested ambiguous identification, whereas ANI analysis assigned isolate yu01 as Coryne. parakroppenstedtii. The fourth genospecies \"Corynebacterium aliikroppenstedtii\" was identified in CKC. Comparative genomics analysis suggested that the genomic arrangement in CKC was highly conserved. A total of 43 potential virulence genes and 79 species-specific genes were detected. Most genome-based phylogenetic analysis were incapable of resolving the interspecific evolutionary relationships among CKCs. A total of 20 core genes were found to be distinguishable in CKC.
CONCLUSIONS: This study suggested the limited divergence and unavailability of normal single gene-based identification in CKC and questioned the precise species of strains associated with mastitis, identified as Coryne. kroppenstedtii in previous studies. The 20 genes showed potential to enhance the methods for the identification and epidemiological investigation of CKC.
结果:对乳腺标本yu01分离株的基因组进行了测序,收集35个CKC基因组。16SrRNA的分析,rpoB,FusA建议模棱两可的识别,而ANI分析将分离株yu01指定为C.parakpostedtii。在CKC中鉴定出第四个基因物种“恶性棒状杆菌”。比较基因组学分析表明,CKC中的基因组排列高度保守。共检测到43个潜在毒力基因和79个物种特异性基因。大多数基于基因组的系统发育分析无法解决CKC之间的种间进化关系。发现20个核心基因在CKC中是可区分的。
结论:这项研究表明,CKC中基于正常单基因的鉴定存在有限的差异和不可用性,并质疑与乳腺炎相关的菌株的确切种类,在以前的研究中被鉴定为C.kroppentedtii。这20个基因显示出增强CKC鉴定和流行病学调查方法的潜力。