Abstract：
It is critical to explore the effects of electromagnetic field (EMF) on the construction of functional osteochondral tissue, which has shown certain clinical significance for the treatment of osteochondral injury. At present, there are few studies on the effect of the direction of EMF on cells. This study aimed to investigate the effects of EMF coupling on different parameters to control adipose-derived stem cells (ADSCs) proliferation and specific chondrogenic and osteogenic differentiation at 2D level and 3D level. The proliferation and differentiation of EMF-induced ADSCs are jointly regulated by EMF and space structure. In this study, Cs7/Gel3/nHAP scaffolds were prepared with good degradation rate (86.75 ± 4.96 %) and absorb water (1100 %), and the pore size was 195.63 ± 54.72 μm. The bone-derived scaffold with a pore size of 267.17 ± 129.18 μm was obtained and its main component was hydroxyapatite. Cs7/Gel3/nHAP scaffolds and bone-derived scaffolds are suitable as 3D level materials. The optimal EMF intensity was 2 mT for chondrogenic differentiation and proliferation and 1 mT for osteogenic differentiation and proliferation. It is noteworthy that EMF has a negative correlation with ADSCs proliferation in the vertical direction at 2D level, while it has a positive correlation with ADSCs proliferation at 3D level. EMF mediated 3D osteochondral scaffold provide good strategy for osteochondral tissue engineering construction.
摘要：
探索电磁场(EMF)对功能性骨软骨组织构建的影响至关重要,骨软骨损伤的治疗具有一定的临床意义。目前,关于EMF方向对细胞的影响的研究很少。本研究旨在探讨EMF偶联对不同参数在2D水平和3D水平上控制脂肪干细胞（ADSCs）增殖和特异性软骨和成骨分化的影响。EMF诱导的ADSCs的增殖和分化受EMF和空间结构共同调控。在这项研究中,制备的Cs7/Gel3/nHAP支架具有良好的降解率(86.75±4.96%)和吸水性(1100%),孔径为195.63±54.72μm。获得了孔径为267.17±129.18μm的骨衍生支架，其主要成分为羟基磷灰石。Cs7/Gel3/nHAP支架和骨源支架适合作为3D级材料。最佳EMF强度为2mT用于软骨分化和增殖，1mT用于成骨分化和增殖。值得注意的是，在2D水平上，EMF与ADSCs在垂直方向上的增殖呈负相关，同时与3D水平的ADSCs增殖呈正相关。EMF介导的3D骨软骨支架为骨软骨组织工程构建提供了良好的策略。
