PDF(Pubmed)
Abstract：
The cold chain conditions have been suggested to facilitate long-distance transmission of SARS-CoV-2, but it is unclear how viable the virus is on cold chain packaging materials.
This study used the MHV-JHM strain of murine hepatitis virus as a model organism to investigate the viability of SARS-CoV-2 on foam, plastic, cardboard, and wood sheets at different temperatures (-40°C, -20°C, and 4°C). In addition, the ability of peracetic acid and sodium hypochlorite to eliminate the MHV-JHM on plastic and cardboard sheets were also evaluated.
The results indicate that MHV-JHM can survive on foam, plastic, or cardboard sheets for up to 28 days at -40°C and -20°C, and up to 14 days on foam and plastic surfaces at 4°C. Although viral nucleic acids were still detectable after storing at 4°C for 28 days, the corresponding virus titer was below the limit of quantification (LOQ).
The study highlights that a positive nucleic acid test result may not indicate that the virus is still viable, and confirms that peracetic acid and sodium hypochlorite can effectively eliminate MHV-JHM on packaging materials under cold chain conditions.
This study used the MHV-JHM strain of murine hepatitis virus as a model organism to investigate the viability of SARS-CoV-2 on foam, plastic, cardboard, and wood sheets at different temperatures (-40°C, -20°C, and 4°C). In addition, the ability of peracetic acid and sodium hypochlorite to eliminate the MHV-JHM on plastic and cardboard sheets were also evaluated.
The results indicate that MHV-JHM can survive on foam, plastic, or cardboard sheets for up to 28 days at -40°C and -20°C, and up to 14 days on foam and plastic surfaces at 4°C. Although viral nucleic acids were still detectable after storing at 4°C for 28 days, the corresponding virus titer was below the limit of quantification (LOQ).
The study highlights that a positive nucleic acid test result may not indicate that the virus is still viable, and confirms that peracetic acid and sodium hypochlorite can effectively eliminate MHV-JHM on packaging materials under cold chain conditions.
摘要：
■已提出冷链条件以促进SARS-CoV-2的远距离传播，但尚不清楚该病毒在冷链包装材料上的可行性。
■本研究以鼠肝炎病毒MHV-JHM株作为模型生物，研究SARS-CoV-2在泡沫上的生存力，塑料,纸板,和不同温度下的木板(-40°C，-20°C,和4°C）。此外,还评估了过乙酸和次氯酸钠消除塑料和纸板上的MHV-JHM的能力。
■结果表明MHV-JHM可以在泡沫上存活，塑料,或纸板在-40°C和-20°C下最多28天，在4°C下在泡沫和塑料表面上长达14天。尽管病毒核酸在4°C储存28天后仍可检测到，相应的病毒滴度低于定量限(LOQ).
■该研究强调，核酸检测阳性结果可能不表明病毒仍然存活，并证实过乙酸和次氯酸钠能有效消除冷链条件下包装材料上的MHV-JHM。
■本研究以鼠肝炎病毒MHV-JHM株作为模型生物，研究SARS-CoV-2在泡沫上的生存力，塑料,纸板,和不同温度下的木板(-40°C，-20°C,和4°C）。此外,还评估了过乙酸和次氯酸钠消除塑料和纸板上的MHV-JHM的能力。
■结果表明MHV-JHM可以在泡沫上存活，塑料,或纸板在-40°C和-20°C下最多28天，在4°C下在泡沫和塑料表面上长达14天。尽管病毒核酸在4°C储存28天后仍可检测到，相应的病毒滴度低于定量限(LOQ).
■该研究强调，核酸检测阳性结果可能不表明病毒仍然存活，并证实过乙酸和次氯酸钠能有效消除冷链条件下包装材料上的MHV-JHM。
