Abstract：
We tested the hypothesis that AMPK activation and peroxisome proliferator gamma coactivator 1 alpha (PGC-1α) expression are not augmented as exercise intensity (power output) increases from maximal to supramaximal intensities and conducted an exploratory analysis comparing AMPK activation and PGC-1α expression in males and females. Seventeen (n = 9 males; n = 8 females) recreationally active, healthy, young individuals volunteered to participate in the current study. Participants completed work matched interval exercise at 100% (Max) and 133% (Supra) of peak work rate (WRpeak). Intervals were 1 min in duration and participants were prescribed 6 and 8 intervals of Max and Supra, respectively, to equate external work across protocols. PGC-1α mRNA expression and activation of AMPK (p-ACC) were examined in muscle biopsy samples. Interval WR (watts; W), intensity (%WRpeak) and average HR (bpm), blood lactate (mmol/L) and rating of perceived exertion were all higher (all p < 0.05) in Supra. Fatigue was greater (p < 0.05) in Supra. PGC-1α mRNA expression significantly increased after exercise in Max (p < 0.01) and Supra (p < 0.01), but was not significantly different (p = 0.71) between intensities. A main effect of time (Pre - 0 h) (p < 0.01) was observed for p-ACC; however, no effect of intensity (p = 0.08) or interaction (p = 0.97) was observed. No significant effects of time (p = 0.05) intensity (p = 0.42), or interaction (p = 0.97) were observed for p-AMPK (Thr172). Exploratory sex analysis demonstrated a main effect of sex for p-ACC (greater p-ACC in males; p < 0.05) but not for p-AMPK or PGC-1α expression. Our results confirm that AMPK-PGC-1α signalling is not augmented following supramaximal exercise and provide novel data demonstrating a decrease in AMPK activation (p-ACC) in females compared to men. Trial registration: https://doi.org/10.17605/OSF.IO/U7PX9.
摘要：
我们测试了以下假设：随着运动强度（功率输出）从最大强度增加到超最大强度，AMPK激活和PGC-1α表达没有增加，并进行了探索性分析，比较了男性和女性的AMPK激活和PGC-1α表达。
方法：17（n=9男性；n=8女性）娱乐活动，健康,年轻人自愿参加当前的研究。参与者以峰值工作率[WRpeak]的100%[Max]和133%[Supra]完成了与间歇锻炼相匹配的工作。间隔时间为1分钟，参与者分别为Max和Supra规定了6和8个间隔时间，以等同于跨协议的外部工作。在肌肉活检样品中检查了PGC-1αmRNA的表达和AMPK（p-ACC）的激活。
结果：间隔WR（瓦特；W），强度(%WRpeak)和平均HR(BPM)，血乳酸（mmol/L）和RPE均较高（均p<0.05）。Supra的疲劳更大（p<0.05）。运动后PGC-1αmRNA表达显著增加(p<0.01),但强度之间没有显着差异（p=0.71）。对于p-ACC，观察到时间的主要影响（Pre-0h）（p<0.01）然而,没有观察到强度(p=0.08)或相互作用(p=0.97)的影响.时间(p=0.05)强度(p=0.42)没有显著影响，或相互作用(p=0.97)观察到p-AMPK(Thr172)。探索性性别分析表明，性别对p-ACC的主要影响（男性中p-ACC更大；p<0.05），但对p-AMPK或PGC-1α表达没有影响。
结论：我们的结果证实，AMPK-PGC-1α信号在超运动后没有增强，并提供了新的数据表明，与男性相比，女性的AMPK激活（p-ACC）降低。
方法：17（n=9男性；n=8女性）娱乐活动，健康,年轻人自愿参加当前的研究。参与者以峰值工作率[WRpeak]的100%[Max]和133%[Supra]完成了与间歇锻炼相匹配的工作。间隔时间为1分钟，参与者分别为Max和Supra规定了6和8个间隔时间，以等同于跨协议的外部工作。在肌肉活检样品中检查了PGC-1αmRNA的表达和AMPK（p-ACC）的激活。
结果：间隔WR（瓦特；W），强度(%WRpeak)和平均HR(BPM)，血乳酸（mmol/L）和RPE均较高（均p<0.05）。Supra的疲劳更大（p<0.05）。运动后PGC-1αmRNA表达显著增加(p<0.01),但强度之间没有显着差异（p=0.71）。对于p-ACC，观察到时间的主要影响（Pre-0h）（p<0.01）然而,没有观察到强度(p=0.08)或相互作用(p=0.97)的影响.时间(p=0.05)强度(p=0.42)没有显著影响，或相互作用(p=0.97)观察到p-AMPK(Thr172)。探索性性别分析表明，性别对p-ACC的主要影响（男性中p-ACC更大；p<0.05），但对p-AMPK或PGC-1α表达没有影响。
结论：我们的结果证实，AMPK-PGC-1α信号在超运动后没有增强，并提供了新的数据表明，与男性相比，女性的AMPK激活（p-ACC）降低。
