Abstract：
Platelet-rich plasma (PRP) has significant potential for various applications and holds clinical value in regenerative medicine. Cryopreservation is used to extend the preservation period of PRP, facilitating its clinical application. However, the potential negative effects of long-term cryopreservation on platelet storage lesion are still uncertain. In this study, PRP was stored at - 30 °C or - 80 °C. Platelet count, apoptosis, reactive oxygen species (ROS) content, and CD62P expression were assessed on the 14th and 28th days. The study also evaluated platelet mitochondria morphology and function, serotonin (5-HT) secretion by platelets, and the inflammatory activating effect of cryopreserved platelets in PRP. The results showed that there were no significant differences in platelet count, the content of 5-HT, and inflammatory effects between fresh PRP and PRP cryopreserved at both - 30 °C and - 80 °C. However, there was an increase in ROS level, apoptosis, and CD62P level after cryopreservation at both temperatures. Additionally, the levels of ROS, apoptosis, and CD62P in platelets were similar after storage at - 30 °C and - 80 °C. The main difference observed was that the morphology and function of mitochondria were severely damaged after storage at - 30 °C, while they were less affected at - 80 °C. Based on these findings, it can be concluded that storing PRP at - 80 °C is more suitable for achieving a better therapeutic effect in clinical applications, but cryopreservation could not replace the current standard.
摘要：
富血小板血浆（PRP）在各种应用中具有重要的潜力，在再生医学中具有临床价值。冷冻保存用于延长PRP的保存期限，促进其临床应用。然而,长期冷冻保存对血小板储存损伤的潜在负面影响仍不确定.在这项研究中,PRP储存在-30°C或-80°C。血小板计数,凋亡，活性氧（ROS）含量，在第14天和第28天评估CD62P表达。该研究还评估了血小板线粒体形态和功能，血小板分泌5-羟色胺（5-HT），以及冷冻保存的血小板在PRP中的炎症激活作用。结果显示，血小板计数无显著差异，5-HT的含量，以及在-30°C和-80°C下冷冻保存的新鲜PRP和PRP之间的炎症作用。然而,ROS水平增加了，凋亡，在两个温度下冷冻保存后的CD62P水平。此外,ROS的水平，凋亡，在-30°C和-80°C储存后，血小板中的CD62P相似。观察到的主要差异是线粒体在-30°C储存后的形态和功能受到严重破坏，而它们在-80°C时受到的影响较小。基于这些发现,可以得出结论，将PRP储存在-80°C更适合在临床应用中获得更好的治疗效果，但冷冻保存不能代替现行标准。
