Abstract：
Polygonum multiflorum Thunb (PM) is used to slow the aging process. Although polysaccharides are a major constituent of PM, their anti-aging properties have not been thoroughly investigated. Therefore, this study aimed to examine the anti-aging effects of polysaccharides extracted from PM using the Caenorhabditis elegans (C. elegans) model. Two types of water-soluble heteropolysaccharides, namely a neutral polysaccharide (RPMP-N) and an acidic polysaccharide (RPMP-A), were obtained from PM. Their structures were elucidated by various methods. The effects of these polysaccharides on the lifespan, levels of antioxidants, and activities of antioxidant-related enzymes in C. elegans were also evaluated. The results showed that RPMP-A had higher GalA content compared with RPMP-N. The average molecular weights of RPMP-N and RPMP-A were 245.30 and 28.45 kDa, respectively. RPMP-N is a α-1,4-linked dextran as the main chain, and contains a small amount of branched dextran with O-6 as the branched linkage site；RPMP-A may be a complex of α-1,4-linked dextran, HG and RG-I. Treatment with RPMP-N and RPMP-A increased the mean lifespan of C. elegans, and significantly regulated oxidative stress. RPMP-A exhibited stronger anti-aging effects compared with RPMP-N. These findings suggest that RPMP-A may be a potent antioxidant and anti-aging component that can be used for developing functional food products and effective dietary supplements.
摘要：
何首乌（PM）用于减缓衰老过程。虽然多糖是PM的主要成分，它们的抗老化性能尚未得到彻底研究。因此,这项研究旨在研究使用秀丽隐杆线虫从PM中提取的多糖的抗衰老作用（C.线虫）模型。两种水溶性杂多糖，即中性多糖（RPMP-N）和酸性多糖（RPMP-A），是从PM获得的。通过各种方法阐明了它们的结构。这些多糖对寿命的影响，抗氧化剂的水平，还评估了秀丽隐杆线虫中抗氧化相关酶的活性。结果表明，与RPMP-N相比，RPMP-A具有更高的GalA含量。RPMP-N和RPMP-A的平均分子量分别为245.30和28.45kDa，分别。RPMP-N是α-1,4-连接的葡聚糖作为主链,并含有少量以O-6为支链连接位点的支链葡聚糖；RPMP-A可能是α-1,4-连接葡聚糖的复合物，HG和RG-I用RPMP-N和RPMP-A治疗可增加秀丽隐杆线虫的平均寿命，并显著调节氧化应激。与RPMP-N相比，RPMP-A具有更强的抗衰老作用。这些发现表明，RPMP-A可能是一种有效的抗氧化剂和抗衰老成分，可用于开发功能性食品和有效的膳食补充剂。
