PDF(Pubmed)
Abstract：
While bioorthogonal reactions are routinely employed in living cells and organisms, their application within individual organelles remains limited. In this review, we highlight diverse examples of bioorthogonal reactions used to investigate the roles of biomolecules and biological processes as well as advanced imaging techniques within cellular organelles. These innovations hold great promise for therapeutic interventions in personalized medicine and precision therapies. We also address existing challenges related to the selectivity and trafficking of subcellular dynamics. Organelle-targeted bioorthogonal reactions have the potential to significantly advance our understanding of cellular organization and function, provide new pathways for basic research and clinical applications, and shape the direction of cell biology and medical research.
摘要：
虽然生物正交反应通常用于活细胞和生物体，它们在单个细胞器中的应用仍然有限。在这次审查中,我们重点介绍了用于研究生物分子和生物过程的作用以及细胞器中先进的成像技术的生物正交反应的各种例子。这些创新为个性化医学和精准治疗的治疗干预提供了巨大的希望。我们还解决了与亚细胞动力学的选择性和贩运有关的现有挑战。细胞器靶向的生物正交反应有可能显著提高我们对细胞组织和功能的理解，为基础研究和临床应用提供新途径，塑造细胞生物学和医学研究的方向。
