PDF(Pubmed)
Abstract：
Giardia duodenalis is a common pathogenic intestinal protozoan parasite with high prevalence in developing countries, especially among children. The distribution of giardia assemblages among humans and their clinical relevance remains controversial. This study aimed to determine the prevalence and assemblage of Giardia among children under 5 years of age in Jimma, Southwest Ethiopia. Employing a case-control design, 606 children presenting with diarrhea at Jimma university medical center and Serbo Health Center were enrolled from December 2016 to July 2018 along with 617 matched controls without diarrhea. Giardia was detected and typed using real-time PCR. Univariate and multivariate regression analysis was performed. The total prevalence of Giardia was 41% (501/1223) and did not differ significantly between cases and controls (40% vs 42%). Prevalence increased by age, with the highest prevalence seen in children aged ≥ 25 months. Children without diarrhea with a history of diarrhea during the last month were more likely to be Giardia positive compared to children with no history diarrhea (OR 1.8 and 95%CI; 1.1-2.9). Regardless of current diarrhea symptoms, assemblage B predominated with 89%, followed by assemblage A (8%) and mixed infection assemblage A and B (3%). We report a high prevalence of Giardia by PCR detection in Jimma, Ethiopia, with assemblage B being predominant. There was a similar distribution of Giardia assemblages between children with and without diarrhea. Increasing age was a risk factor for Giardia infection. Community-based prevention and control strategies need to be employed to decrease the risk of giardia infection.
摘要：
十二指肠贾第鞭毛虫是一种常见的致病性肠道原虫寄生虫，在发展中国家患病率较高。尤其是在儿童中。贾第鞭毛虫组合在人类中的分布及其临床相关性仍存在争议。这项研究旨在确定Jimma5岁以下儿童中贾第虫的患病率和聚集情况。埃塞俄比亚西南部。采用案例控制设计，从2016年12月至2018年7月，在Jimma大学医学中心和Serbo健康中心出现腹泻的606名儿童以及617名没有腹泻的匹配对照。使用实时PCR检测和分型贾第虫。进行单因素和多因素回归分析。贾第虫的总患病率为41％（501/1223），病例和对照组之间没有显着差异（40％vs42％）。患病率随年龄增加，在≥25个月的儿童中患病率最高。与没有腹泻史的儿童相比，上个月没有腹泻史的儿童更可能是贾第鞭毛虫阳性（OR1.8和95CI;1.1-2.9）。不管目前的腹泻症状如何，组合B占89%，其次是组合A（8％）和混合感染组合A和B（3％）。我们报告了在Jimma通过PCR检测发现贾第虫的高患病率，埃塞俄比亚,组合B占主导地位。有和没有腹泻的儿童之间贾第虫组合的分布相似。年龄增长是贾第虫感染的危险因素。需要采用基于社区的预防和控制策略来降低贾第虫感染的风险。
