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Abstract：
UNASSIGNED: Cholera is an acute intestinal infection caused by Vibrio cholera (V. cholera). The development of antibodies against specific V. cholerae may have a therapeutic effect. In the present research, we investigated the protective effect of egg yolk Immunoglobulin (IgY), which was produced by immunizing hens with formaldehyde-killed V. cholerae O1 and subsequently the isolated IgY was orally administrated to the V. cholerae O1 infected mice for evaluation of its immunizing capability.
UNASSIGNED: In the current study, hens were immunized three times with formaldehyde-killed V. cholerae O1 (1.5×107 CFU/ml) and an equal volume of adjuvant. The IgY was isolated from egg yolk by polyethylene glycol method. The validity and activity of isolated IgY were confirmed with SDS-PAGE and ELISA methods, respectively. Subsequently IgY was orally administered to suckling mice following challenge with V. cholerae O1. ELISA results showed high antibody titer in the serum and egg yolk. Also, SDS-PAGE analysis showed successful purification of IgY and anti-V. cholerae IgY prevented the death of mice infected with V. cholerae O1. The anti-V. cholera IgY was administered at 2, 4, 6 hr intervals after 3 hr of inoculation of mice with V. cholerae O1.
UNASSIGNED: Results showed that the rate of surviving mice (2 mg/ml of IgY) were 60% after 4 hr and 40% after 6 hr and the rate of surviving mice (5 mg/ml of IgY) was 70% after 4 hr and 60% after 6 hr.
UNASSIGNED: The findings suggested the egg yolk-driven IgY as a natural antibacterial protein, could be effective in the prevention and treatment of cholera disease.
UNASSIGNED: In the current study, hens were immunized three times with formaldehyde-killed V. cholerae O1 (1.5×107 CFU/ml) and an equal volume of adjuvant. The IgY was isolated from egg yolk by polyethylene glycol method. The validity and activity of isolated IgY were confirmed with SDS-PAGE and ELISA methods, respectively. Subsequently IgY was orally administered to suckling mice following challenge with V. cholerae O1. ELISA results showed high antibody titer in the serum and egg yolk. Also, SDS-PAGE analysis showed successful purification of IgY and anti-V. cholerae IgY prevented the death of mice infected with V. cholerae O1. The anti-V. cholera IgY was administered at 2, 4, 6 hr intervals after 3 hr of inoculation of mice with V. cholerae O1.
UNASSIGNED: Results showed that the rate of surviving mice (2 mg/ml of IgY) were 60% after 4 hr and 40% after 6 hr and the rate of surviving mice (5 mg/ml of IgY) was 70% after 4 hr and 60% after 6 hr.
UNASSIGNED: The findings suggested the egg yolk-driven IgY as a natural antibacterial protein, could be effective in the prevention and treatment of cholera disease.
摘要：
■霍乱是由霍乱弧菌（V.霍乱)。针对特定霍乱弧菌的抗体的开发可能具有治疗作用。在目前的研究中,我们研究了卵黄免疫球蛋白（IgY）的保护作用，它是通过用甲醛杀死的霍乱弧菌O1免疫母鸡而产生的，随后将分离的IgY口服施用于霍乱弧菌O1感染的小鼠，以评估其免疫能力。
■在当前的研究中，用甲醛杀死的霍乱弧菌O1（1.5×107CFU/ml）和等体积的佐剂免疫母鸡3次。采用聚乙二醇法从蛋黄中分离IgY。用SDS-PAGE和ELISA方法证实了分离的IgY的有效性和活性。分别。随后,在用霍乱弧菌O1攻击后,将IgY口服施用于乳鼠。ELISA成果显示血清和卵黄中抗体效价较高。此外，SDS-PAGE分析表明成功纯化了IgY和抗V。霍乱IgY可预防感染霍乱弧菌O1的小鼠死亡。反V用霍乱弧菌O1接种小鼠3小时后，每隔2、4、6小时给予霍乱IgY。
结果显示,4小时后存活小鼠(2mg/mlIgY)的比率为60%,6小时后存活小鼠(5mg/mlIgY)的比率为40%,4小时后存活小鼠(5mg/mlIgY)的比率为70%,6小时后存活小鼠的比率为60%。
■研究结果表明，蛋黄驱动的IgY是一种天然的抗菌蛋白，可以有效地预防和治疗霍乱疾病。
■在当前的研究中，用甲醛杀死的霍乱弧菌O1（1.5×107CFU/ml）和等体积的佐剂免疫母鸡3次。采用聚乙二醇法从蛋黄中分离IgY。用SDS-PAGE和ELISA方法证实了分离的IgY的有效性和活性。分别。随后,在用霍乱弧菌O1攻击后,将IgY口服施用于乳鼠。ELISA成果显示血清和卵黄中抗体效价较高。此外，SDS-PAGE分析表明成功纯化了IgY和抗V。霍乱IgY可预防感染霍乱弧菌O1的小鼠死亡。反V用霍乱弧菌O1接种小鼠3小时后，每隔2、4、6小时给予霍乱IgY。
结果显示,4小时后存活小鼠(2mg/mlIgY)的比率为60%,6小时后存活小鼠(5mg/mlIgY)的比率为40%,4小时后存活小鼠(5mg/mlIgY)的比率为70%,6小时后存活小鼠的比率为60%。
■研究结果表明，蛋黄驱动的IgY是一种天然的抗菌蛋白，可以有效地预防和治疗霍乱疾病。
