Abstract：
Biofilm formation by Pseudomonas aeruginosa is primarily responsible for chronic wound and lung infections in humans. These infections are persistent owing to the biofilm\'s high tolerance to antimicrobials and constantly changing environmental factors. Understanding the mechanism governing biofilm formation can help to develop therapeutics explicitly directed against the molecular markers responsible for this process. After numerous years of research, many genes responsible for both in vitro and in vivo biofilm development remain unidentified. However, there is no \"all in one\" complete in vivo or in vitro biofilm model. Recent findings imply that the shift from planktonic bacteria to biofilms is a complicated and interrelated differentiation process. Research on the applications of omics technologies in P. aeruginosa biofilm development is ongoing, and these approaches hold great promise for expanding our knowledge of the mechanisms of biofilm formation. This review discusses the different factors that affect biofilm formation and compares P. aeruginosa biofilm formation using the omics approaches targeting essential biological macromolecules, such as DNA, RNA, Protein, and metabolome. Furthermore, we have outlined the application of currently available omics tools, such as genomics, proteomics, metabolomics, transcriptomics, and integrated multi-omics methodologies, to understand the differential gene expression (biofilm vs. planktonic bacteria) of P. aeruginosa biofilms.
摘要：
铜绿假单胞菌的生物膜形成是人类慢性伤口和肺部感染的主要原因。由于生物膜对抗菌药物的高耐受性和不断变化的环境因素，这些感染是持续存在的。了解控制生物膜形成的机制可以帮助开发明确针对负责该过程的分子标记的疗法。经过多年的研究,许多负责体外和体内生物膜发育的基因仍未被鉴定。然而,没有完整的体内或体外生物膜模型。最近的发现表明，从浮游细菌到生物膜的转变是一个复杂且相互关联的分化过程。组学技术在铜绿假单胞菌生物膜开发中的应用研究正在进行中，这些方法对扩大我们对生物膜形成机制的认识大有希望。这篇综述讨论了影响生物膜形成的不同因素，并使用针对基本生物大分子的组学方法比较了铜绿假单胞菌生物膜的形成。比如DNA,RNA,蛋白质,和代谢组。此外，我们已经概述了当前可用的组学工具的应用，比如基因组学，蛋白质组学，代谢组学，转录组学，和综合的多组学方法，了解差异基因表达(生物膜与浮游细菌）铜绿假单胞菌生物膜。
