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Abstract：
This study presents an in vitro analysis of the bactericidal and cytotoxic properties of hybrid films containing nickel oxide (NiO) and nickel ferrite (NiFe2O4) nanoparticles embedded in polypropylene (PP). The solvent casting method was used to synthesize films of PP, PP@NiO, and PP@NiFe2O4, which were characterized by different spectroscopic and microscopic techniques. The X-ray diffraction (XRD) patterns confirmed that the small crystallite sizes of NiO and NiFe2O4 NPs were maintained even after they were incorporated into the PP matrix. From the Raman scattering spectroscopy data, it was evident that there was a significant interaction between the NPs and the PP matrix. Additionally, the Scanning Electron Microscopy (SEM) analysis revealed a homogeneous dispersion of NiO and NiFe2O4 NPs throughout the PP matrix. The incorporation of the NPs was observed to alter the surface roughness of the films; this behavior was studied by atomic force microscopy (AFM). The antibacterial properties of all films were evaluated against Pseudomonas aeruginosa (ATCC®: 43636™) and Staphylococcus aureus (ATCC®: 23235™), two opportunistic and nosocomial pathogens. The PP@NiO and PP@ NiFe2O4 films showed over 90% bacterial growth inhibition for both strains. Additionally, the effects of the films on human skin cells, such as epidermal keratinocytes and dermal fibroblasts, were evaluated for cytotoxicity. The PP, PP@NiO, and PP@NiFe2O4 films were nontoxic to human keratinocytes. Furthermore, compared to the PP film, improved biocompatibility of the PP@NiFe2O4 film with human fibroblasts was observed. The methodology utilized in this study allows for the production of hybrid films that can inhibit the growth of Gram-positive bacteria, such as S. aureus, and Gram-negative bacteria, such as P. aeruginosa. These films have potential as coating materials to prevent bacterial proliferation on surfaces.
摘要：
这项研究提出了含有嵌入聚丙烯（PP）中的氧化镍（NiO）和镍铁氧体（NiFe2O4）纳米颗粒的杂化膜的杀菌和细胞毒性的体外分析。采用溶剂流延法合成PP薄膜,PP@NiO,和PP@NiFe2O4，通过不同的光谱和显微技术进行了表征。X射线衍射（XRD）图证实，即使将NiO和NiFe2O4NP掺入PP基质中，它们仍保持着小的微晶尺寸。从拉曼散射光谱数据来看，很明显，NP和PP基质之间存在显著的相互作用。此外,扫描电子显微镜（SEM）分析显示NiO和NiFe2O4NP在整个PP基质中的均匀分散。观察到NPs的掺入改变了薄膜的表面粗糙度；通过原子力显微镜（AFM）研究了这种行为。对所有薄膜的抗菌性能进行评估，针对铜绿假单胞菌(ATCC®:43636™)和金黄色葡萄球菌(ATCC®:23235™)，两种机会性和院内病原体。PP@NiO和PP@NiFe2O4膜对两种菌株均显示出超过90%的细菌生长抑制。此外,这些薄膜对人体皮肤细胞的影响，如表皮角质形成细胞和真皮成纤维细胞，评估细胞毒性。PP,PP@NiO,PP@NiFe2O4膜对人角质形成细胞无毒。此外，与PP薄膜相比，观察到PP@NiFe2O4膜与人成纤维细胞的生物相容性改善。本研究中使用的方法允许生产可以抑制革兰氏阳性细菌生长的杂化膜，如金黄色葡萄球菌，和革兰氏阴性细菌，例如铜绿假单胞菌。这些膜具有作为涂层材料以防止表面上的细菌增殖的潜力。
