METHODS: Artiodactyl feces were collected between 2020 and 2021. The fecal samples were examined to identify the host through macroscopic and molecular analysis. The remaining samples were subjected to a fecal parasite analysis using microscopic techniques, and feces containing cysts of the phylum Ciliophora underwent a molecular analysis.
RESULTS: Of the 101 samples collected, 71.3% were found in Pavão Valley, the most anthropized area of the park. In the molecular analysis, 79 samples were identified as belonging to Sus scrofa and 2 as Mazama gouazoubira. The most frequently detected forms were cysts of the phylum Ciliophora (39.6%), followed by eggs of Ascaris spp. (11.8%) and Metastrongylus spp. (5.9%). Nucleotide sequences of Balantioides coli were characterized in 26 samples, and in 13 samples variants of type B0 and in 11 type A0 were identified.
CONCLUSIONS: It should be noted that this is the first study in the Americas that has identified B. coli in free-living S. scrofa feces, citing this bioinvader as one of the reservoirs of this parasite.
方法:收集了2020年至2021年之间的偶蹄粪便。通过宏观和分子分析检查粪便样品以鉴定宿主。使用显微镜技术对剩余的样品进行粪便寄生虫分析,并对含有Ciliophora门囊肿的粪便进行了分子分析。
结果:在收集的101个样本中,71.3%的人在帕瓦山谷被发现,公园里最人性化的区域.在分子分析中,79个样品被鉴定为Susscrofa,2个样品被鉴定为Mazamagouazoubira。最常见的形式是纤毛门的囊肿(39.6%),其次是蛔虫卵。(11.8%)和Metacrongylusspp。(5.9%)。在26个样本中鉴定了Balantioidescoli的核苷酸序列,在13个样品中鉴定出B0型变体和11个A0型变体。
结论:应该注意的是,这是美洲首次在自由生活的S.scrofa粪便中鉴定出大肠杆菌的研究,引用这种生物入侵者作为这种寄生虫的水库之一。