PDF(Pubmed)
Abstract：
BACKGROUND: The purpose of this study was to identify the species of artiodactyl host related to the fecal matter collected in a forest area in Rio de Janeiro state and carry out a parasitological investigation.
METHODS: Artiodactyl feces were collected between 2020 and 2021. The fecal samples were examined to identify the host through macroscopic and molecular analysis. The remaining samples were subjected to a fecal parasite analysis using microscopic techniques, and feces containing cysts of the phylum Ciliophora underwent a molecular analysis.
RESULTS: Of the 101 samples collected, 71.3% were found in Pavão Valley, the most anthropized area of the park. In the molecular analysis, 79 samples were identified as belonging to Sus scrofa and 2 as Mazama gouazoubira. The most frequently detected forms were cysts of the phylum Ciliophora (39.6%), followed by eggs of Ascaris spp. (11.8%) and Metastrongylus spp. (5.9%). Nucleotide sequences of Balantioides coli were characterized in 26 samples, and in 13 samples variants of type B0 and in 11 type A0 were identified.
CONCLUSIONS: It should be noted that this is the first study in the Americas that has identified B. coli in free-living S. scrofa feces, citing this bioinvader as one of the reservoirs of this parasite.
METHODS: Artiodactyl feces were collected between 2020 and 2021. The fecal samples were examined to identify the host through macroscopic and molecular analysis. The remaining samples were subjected to a fecal parasite analysis using microscopic techniques, and feces containing cysts of the phylum Ciliophora underwent a molecular analysis.
RESULTS: Of the 101 samples collected, 71.3% were found in Pavão Valley, the most anthropized area of the park. In the molecular analysis, 79 samples were identified as belonging to Sus scrofa and 2 as Mazama gouazoubira. The most frequently detected forms were cysts of the phylum Ciliophora (39.6%), followed by eggs of Ascaris spp. (11.8%) and Metastrongylus spp. (5.9%). Nucleotide sequences of Balantioides coli were characterized in 26 samples, and in 13 samples variants of type B0 and in 11 type A0 were identified.
CONCLUSIONS: It should be noted that this is the first study in the Americas that has identified B. coli in free-living S. scrofa feces, citing this bioinvader as one of the reservoirs of this parasite.
摘要：
背景：这项研究的目的是确定与在里约热内卢州森林地区收集的粪便有关的偶蹄动物宿主的种类，并进行寄生虫学调查。
方法：收集了2020年至2021年之间的偶蹄粪便。通过宏观和分子分析检查粪便样品以鉴定宿主。使用显微镜技术对剩余的样品进行粪便寄生虫分析，并对含有Ciliophora门囊肿的粪便进行了分子分析。
结果：在收集的101个样本中，71.3%的人在帕瓦山谷被发现，公园里最人性化的区域.在分子分析中,79个样品被鉴定为Susscrofa，2个样品被鉴定为Mazamagouazoubira。最常见的形式是纤毛门的囊肿（39.6％），其次是蛔虫卵。（11.8%）和Metacrongylusspp。（5.9%）。在26个样本中鉴定了Balantioidescoli的核苷酸序列，在13个样品中鉴定出B0型变体和11个A0型变体。
结论：应该注意的是，这是美洲首次在自由生活的S.scrofa粪便中鉴定出大肠杆菌的研究，引用这种生物入侵者作为这种寄生虫的水库之一。
方法：收集了2020年至2021年之间的偶蹄粪便。通过宏观和分子分析检查粪便样品以鉴定宿主。使用显微镜技术对剩余的样品进行粪便寄生虫分析，并对含有Ciliophora门囊肿的粪便进行了分子分析。
结果：在收集的101个样本中，71.3%的人在帕瓦山谷被发现，公园里最人性化的区域.在分子分析中,79个样品被鉴定为Susscrofa，2个样品被鉴定为Mazamagouazoubira。最常见的形式是纤毛门的囊肿（39.6％），其次是蛔虫卵。（11.8%）和Metacrongylusspp。（5.9%）。在26个样本中鉴定了Balantioidescoli的核苷酸序列，在13个样品中鉴定出B0型变体和11个A0型变体。
结论：应该注意的是，这是美洲首次在自由生活的S.scrofa粪便中鉴定出大肠杆菌的研究，引用这种生物入侵者作为这种寄生虫的水库之一。
