Abstract：
OBJECTIVE: This in vitro study aimed to compare flexural strength, surface roughness, and biofilm formation of ceramic-reinforced polyetheretherketone (PEEK) with conventionally heat-compressed and milled polymethylmethacrylate (PMMA) denture base materials.
METHODS: Thirty strips (6.4 mm × 10 mm × 3 mm) and 30 discs (10 mm × 1 mm) were fabricated from a heat-compressed PMMA, milled PMMA, and ceramic-reinforced PEEK, 10 each. One surface of each sample was polished to mimic the laboratory procedure for denture base materials. Strips were then subjected to a three-point bend test using a universal testing machine at a crosshead speed of 5.0 mm/min. An optical profilometer was used to assess the Ra value (mm) of the discs on polished and unpolished sides. Biofilm formation behavior was analyzed by measuring the colony-forming unit (CFU)/mL of Candida albicans on the unpolished surface of the discs. One-way ANOVA followed by Tukey multiple comparison tests were used to compare the flexural strength, Ra value, and biofilm formation of the studied materials (a = 0.05).
RESULTS: Ceramic-reinforced PEEK showed significantly higher flexural strength (178.2 ± 3.2 MPa) than milled PMMA (89.6 ± 0.8 MPa; p < 0.001) and heat-compressed PMMA (67.3 ± 5.3 MPa; p < 0.001). Ceramic-reinforced PEEK exhibited a significantly higher Ra value than the other groups on unpolished sides; however, the polishing process significantly reduced the Ra values of all studied groups (p < 0.05). There was no significant difference in C. albicans adhesion among the groups (p < 0.05).
CONCLUSIONS: The flexural strength of tested materials was within acceptable limits for clinical use as a denture base material. Ceramic-reinforced PEEK had the highest surface roughness; however, its similarity in biofilm formation to other groups indicates its clinical acceptability as denture base material.
METHODS: Thirty strips (6.4 mm × 10 mm × 3 mm) and 30 discs (10 mm × 1 mm) were fabricated from a heat-compressed PMMA, milled PMMA, and ceramic-reinforced PEEK, 10 each. One surface of each sample was polished to mimic the laboratory procedure for denture base materials. Strips were then subjected to a three-point bend test using a universal testing machine at a crosshead speed of 5.0 mm/min. An optical profilometer was used to assess the Ra value (mm) of the discs on polished and unpolished sides. Biofilm formation behavior was analyzed by measuring the colony-forming unit (CFU)/mL of Candida albicans on the unpolished surface of the discs. One-way ANOVA followed by Tukey multiple comparison tests were used to compare the flexural strength, Ra value, and biofilm formation of the studied materials (a = 0.05).
RESULTS: Ceramic-reinforced PEEK showed significantly higher flexural strength (178.2 ± 3.2 MPa) than milled PMMA (89.6 ± 0.8 MPa; p < 0.001) and heat-compressed PMMA (67.3 ± 5.3 MPa; p < 0.001). Ceramic-reinforced PEEK exhibited a significantly higher Ra value than the other groups on unpolished sides; however, the polishing process significantly reduced the Ra values of all studied groups (p < 0.05). There was no significant difference in C. albicans adhesion among the groups (p < 0.05).
CONCLUSIONS: The flexural strength of tested materials was within acceptable limits for clinical use as a denture base material. Ceramic-reinforced PEEK had the highest surface roughness; however, its similarity in biofilm formation to other groups indicates its clinical acceptability as denture base material.
摘要：
目的：这项体外研究旨在比较弯曲强度，表面粗糙度,以及使用常规热压缩和研磨的聚甲基丙烯酸甲酯（PMMA）义齿基托材料形成陶瓷增强的PEEK的生物膜。
方法：由热压PMMA制成30条（6.4×10×3mm）和30个圆盘（10×1mm），研磨的PMMA，和陶瓷增强PEEK，每个10个。对每个样品的一个表面进行抛光以模拟义齿基托材料的实验室程序。然后使用通用测试机以5.0mm/min的十字头速度对条带进行3点弯曲测试。使用光学轮廓仪评估盘在抛光和未抛光侧的Ra值(mm)。通过测量盘的未抛光表面上的白色念珠菌的菌落形成单位(CFU)/ml来分析生物膜形成行为。单向方差分析，然后使用Tukey多次比较测试来比较弯曲强度，Ra值,和所研究材料的生物膜形成(a=0.05)。
结果：陶瓷增强的PEEK的弯曲强度（178.2±3.2MPa）明显高于研磨的PMMA（89.6±0.8MPa；P<0.001）和热压缩的PMMA（67.3±5.3MPa；P<0.001）。陶瓷增强的PEEK在未抛光的侧面上表现出明显高于其他组的Ra值；但是，抛光过程显着降低了所有研究组的Ra值（P<0.05）。各组间白色念珠菌粘连差异无统计学意义(P>0.05)。
结论：试验材料的弯曲强度在临床上用作义齿基托材料的可接受范围内。陶瓷增强的PEEK具有最高的表面粗糙度；但是，与其他组的生物膜形成相似，表明其作为义齿基托材料的临床可接受性。本文受版权保护。保留所有权利。
方法：由热压PMMA制成30条（6.4×10×3mm）和30个圆盘（10×1mm），研磨的PMMA，和陶瓷增强PEEK，每个10个。对每个样品的一个表面进行抛光以模拟义齿基托材料的实验室程序。然后使用通用测试机以5.0mm/min的十字头速度对条带进行3点弯曲测试。使用光学轮廓仪评估盘在抛光和未抛光侧的Ra值(mm)。通过测量盘的未抛光表面上的白色念珠菌的菌落形成单位(CFU)/ml来分析生物膜形成行为。单向方差分析，然后使用Tukey多次比较测试来比较弯曲强度，Ra值,和所研究材料的生物膜形成(a=0.05)。
结果：陶瓷增强的PEEK的弯曲强度（178.2±3.2MPa）明显高于研磨的PMMA（89.6±0.8MPa；P<0.001）和热压缩的PMMA（67.3±5.3MPa；P<0.001）。陶瓷增强的PEEK在未抛光的侧面上表现出明显高于其他组的Ra值；但是，抛光过程显着降低了所有研究组的Ra值（P<0.05）。各组间白色念珠菌粘连差异无统计学意义(P>0.05)。
结论：试验材料的弯曲强度在临床上用作义齿基托材料的可接受范围内。陶瓷增强的PEEK具有最高的表面粗糙度；但是，与其他组的生物膜形成相似，表明其作为义齿基托材料的临床可接受性。本文受版权保护。保留所有权利。
