Abstract：
To investigate the efficacy of fat grafting in primary tendon healing through immunohistochemical and biomechanical examinations.
The study material comprised a total of 10 male Sprague-Dawley rats, each approximately 10 weeks old. All 10 rats were operated on bilaterally. The right Achilles tendon in all the animals was defined as the study group. The tendon was cut and then repaired, and then fat graft was applied to the repair area. The left Achilles tendon of all the rats constituted the control group. The tendon was cut and repaired with no further application. After 4 weeks, the rats were euthanised and samples were taken from the tendons for immunohistochemical and biomechanical examinations.
In the biomechanical evaluations, no statistically significant difference was determined between the groups in respect of peak load and stiffness values (p: .068, p: .089, respectively). In the histopathological evaluation, the tenocyte value of the study group was superior to that of the control group (p: .04). No statistically significant differences were determined between the groups in respect of the other histopathological parameters. In the immunohistochemical evaluations, the type I collagen and TGF values of the study group were found to be higher than those of the control group (p: .011, p: .012, respectively).
Compared to stem cell applications, the use of fat grafting is clinically easy to apply, has low costs, and has been shown to contribute to tendon healing at an immunohistochemical level with increased collagen and TGF beta values.
The study material comprised a total of 10 male Sprague-Dawley rats, each approximately 10 weeks old. All 10 rats were operated on bilaterally. The right Achilles tendon in all the animals was defined as the study group. The tendon was cut and then repaired, and then fat graft was applied to the repair area. The left Achilles tendon of all the rats constituted the control group. The tendon was cut and repaired with no further application. After 4 weeks, the rats were euthanised and samples were taken from the tendons for immunohistochemical and biomechanical examinations.
In the biomechanical evaluations, no statistically significant difference was determined between the groups in respect of peak load and stiffness values (p: .068, p: .089, respectively). In the histopathological evaluation, the tenocyte value of the study group was superior to that of the control group (p: .04). No statistically significant differences were determined between the groups in respect of the other histopathological parameters. In the immunohistochemical evaluations, the type I collagen and TGF values of the study group were found to be higher than those of the control group (p: .011, p: .012, respectively).
Compared to stem cell applications, the use of fat grafting is clinically easy to apply, has low costs, and has been shown to contribute to tendon healing at an immunohistochemical level with increased collagen and TGF beta values.
摘要：
目的：通过免疫组织化学和生物力学检查，探讨脂肪移植在原发性肌腱愈合中的作用。
方法：研究材料包括10只雄性Sprague-Dawley大鼠，每个大约10周大。全部10只大鼠进行双侧手术。将所有动物的右跟腱定义为研究组。肌腱被切断，然后修复，然后将脂肪移植物应用于修复区域。所有大鼠的左跟腱构成对照组。在没有进一步应用的情况下切割并修复肌腱。4周后,对大鼠实施安乐死，并从肌腱取样进行免疫组织化学和生物力学检查。
结果：在生物力学评估中，在峰值载荷和刚度值(分别为p:.068,p:.089)方面,两组间无统计学显著性差异.在组织病理学评估中，研究组的腱细胞值优于对照组（p:.04）。在其他组织病理学参数方面，两组之间没有统计学上的显着差异。在免疫组织化学评估中，发现研究组的I型胶原和TGF值高于对照组（分别为p：.011，p：.012）。
结论：与干细胞应用相比，使用脂肪移植在临床上很容易应用，成本低,并且已被证明在免疫组织化学水平上有助于肌腱愈合，胶原蛋白和TGFβ值增加。
方法：研究材料包括10只雄性Sprague-Dawley大鼠，每个大约10周大。全部10只大鼠进行双侧手术。将所有动物的右跟腱定义为研究组。肌腱被切断，然后修复，然后将脂肪移植物应用于修复区域。所有大鼠的左跟腱构成对照组。在没有进一步应用的情况下切割并修复肌腱。4周后,对大鼠实施安乐死，并从肌腱取样进行免疫组织化学和生物力学检查。
结果：在生物力学评估中，在峰值载荷和刚度值(分别为p:.068,p:.089)方面,两组间无统计学显著性差异.在组织病理学评估中，研究组的腱细胞值优于对照组（p:.04）。在其他组织病理学参数方面，两组之间没有统计学上的显着差异。在免疫组织化学评估中，发现研究组的I型胶原和TGF值高于对照组（分别为p：.011，p：.012）。
结论：与干细胞应用相比，使用脂肪移植在临床上很容易应用，成本低,并且已被证明在免疫组织化学水平上有助于肌腱愈合，胶原蛋白和TGFβ值增加。
