PDF(Pubmed)
Abstract：
OBJECTIVE: Cheatgrass is one of North America\'s most problematic invasive species. Invasion by this annual grass alters ecosystem structure and function and has proven very challenging to remove with traditional approaches. Commercially available bioherbicides, like P. fluorescens D7, are applied with the goal of providing lasting control from a single application. However, experimental results suggest that this bioherbicide has limited efficacy under field conditions. Potential explanations for variable efficacy include a failure of this bioherbicide to establish in the soil microbiome. However, to our knowledge, no data exist to support or refute this hypothesis. Here, we use a deep-sequencing approach to better understand the effects of this bioherbicide on the soil microbiome and screen for P. fluorescens at 18 months post-application.
摘要：
目的：Cheatgrass是北美最具问题的入侵物种之一。这种一年生草的入侵改变了生态系统的结构和功能，并且已经证明用传统方法去除非常具有挑战性。市售生物除草剂，例如荧光假单胞菌D7,其应用的目的是提供来自单一应用的持久控制。然而,实验结果表明，这种生物除草剂在田间条件下的功效有限。对可变功效的潜在解释包括这种生物除草剂未能在土壤微生物组中建立。然而,根据我们的知识，没有数据支持或反驳这一假设。这里,我们使用深度测序方法来更好地了解这种生物除草剂对土壤微生物组的影响，并在施用后18个月筛选荧光假单胞菌D7。
