Abstract:
Nucleic acid detection is an important tool for clinical diagnosis. The purification of the sample is the most time-consuming step in the nucleic acid testing process and will affect the results of the assay. Here, we developed a surface modification-based nucleic acid purification method and designed an accompanying set of centrifugation equipment and chips to integrate the steps of nucleic acid purification on a single platform. The results of experiments with HeLa cells and HPV type 16 as samples showed that the mentioned method had good nucleic acid purification capability and the accompanying equipment greatly simplified the operation of the experimenters in the whole process. Overall, our equipment can improve the efficiency of nucleic acid purification and is suitable for application in larger-scale clinical assays.
摘要:
核酸检测是临床诊断的重要工具。样品的纯化是核酸检测过程中最耗时的步骤,会影响检测结果。这里,我们开发了一种基于表面修饰的核酸纯化方法,并设计了一套随附的离心设备和芯片,以将核酸纯化的步骤整合在一个平台上。以HeLa细胞和HPV16型为样品的实验结果表明,该方法具有良好的核酸纯化能力,配套设备大大简化了实验人员在整个过程中的操作。总的来说,我们的设备可以提高核酸纯化的效率,适用于更大规模的临床检测。