Abstract：
A case of suspected food poisoning related to the consumption of raw meat from a common minke whale (Balaenoptera acutorostrata) was reported in Tokyo, Japan, in June 2020. Microscopic analysis revealed tissue cysts of Toxoplasma gondii and sarcocysts of Sarcocystis sp. in whale meat. The SAG2 and ITS1 region sequences of T. gondii were detected in the DNA extracted from the meat. Genotyping of the multilocus nested PCR-RFLP using the genetic markers SAG1, SAG2 (5\'- SAG2, 3\'-SAG2, and alt. SAG2), SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico revealed that the genotype of T. gondii was type II, with a type I pattern for the L358 locus. In the phylogenetic analyses of the six loci (GRA6, GRA7, SAG1, HP2, UPRT1, and UPRT7), these sequences clustered into haplogroup 2. Moreover, the sequences of the virulence-related genes ROP5 and ROP18 of T. gondii isolated from whale meat were similar to those of the type II ME49 reference strain. Sequence analyses of the mtDNA cox1 gene, 18S rRNA gene, and ITS1 region indicated the highest similarity of sarcocyst isolated from whale meat to Sarcocystis species that infect birds or carnivores as intermediate hosts; however, the species could not be identified. To our knowledge, this is the first report of T. gondii and Sarcocystis spp. being detected in same whale meat ingested by patients involved in a suspected food poisoning case in Japan.
摘要：
据报道，东京发生了一起与食用普通小须鲸（Balaenopteraacutostrata）生肉有关的疑似食物中毒案件，Japan,2020年6月。显微镜分析显示弓形虫的组织囊肿和Sarcocystissp。鲸鱼肉。在从肉中提取的DNA中检测到弓形虫的SAG2和ITS1区序列。使用遗传标记SAG1，SAG2（5'-SAG2，3'-SAG2和alt。SAG2),SAG3,BTUB,GRA6，c22-8，c29-2，L358，PK1和Apico揭示弓形虫的基因型为II型，具有L358基因座的I型模式。在六个基因座（GRA6，GRA7，SAG1，HP2，UPRT1和UPRT7）的系统发育分析中，这些序列聚集到单倍群2中。此外，从鲸鱼肉中分离出的弓形虫的毒力相关基因ROP5和ROP18的序列与II型ME49参考菌株相似。mtDNAcox1基因的序列分析，18SrRNA基因，和ITS1区域表明从鲸鱼肉中分离出的肌囊与作为中间宿主感染鸟类或食肉动物的肉囊虫物种的相似性最高；然而，该物种无法识别。据我们所知,这是弓形虫和肉囊虫的第一份报告。在日本一起疑似食物中毒案件的患者摄入的同一鲸鱼肉中被检测到。
