PDF(Pubmed)
Abstract：
BACKGROUND: Zostera marina L., or eelgrass, is the most widespread seagrass species throughout the temperate northern hemisphere. Unlike the dry seeds of terrestrial plants, eelgrass seeds must survive in water, and salinity is the key factor influencing eelgrass seed germination. In the present study, transcriptome and proteome analysis were combined to investigate the mechanisms via which eelgrass seed germination was stimulated by low salinity, in addition to the dynamics of key metabolic pathways under germination.
RESULTS: According to the results, low salinity stimulated the activation of Ca2+ signaling and phosphatidylinositol signaling, and further initiated various germination-related physiological processes through the MAPK transduction cascade. Starch, lipids, and storage proteins were mobilized actively to provide the energy and material basis for germination; abscisic acid synthesis and signal transduction were inhibited whereas gibberellin synthesis and signal transduction were activated, weakening seed dormancy and preparing for germination; cell wall weakening and remodeling processes were activated to provide protection for cotyledon protrusion; in addition, multiple antioxidant systems were activated to alleviate oxidative stress generated during the germination process; ERF transcription factor has the highest number in both stages suggested an active role in eelgrass seed germination.
CONCLUSIONS: In summary, for the first time, the present study investigated the mechanisms by which eelgrass seed germination was stimulated by low salinity and analyzed the transcriptomic and proteomic features during eelgrass seed germination comprehensively. The results of the present study enhanced our understanding of seagrass seed germination, especially the molecular ecology of seagrass seeds.
RESULTS: According to the results, low salinity stimulated the activation of Ca2+ signaling and phosphatidylinositol signaling, and further initiated various germination-related physiological processes through the MAPK transduction cascade. Starch, lipids, and storage proteins were mobilized actively to provide the energy and material basis for germination; abscisic acid synthesis and signal transduction were inhibited whereas gibberellin synthesis and signal transduction were activated, weakening seed dormancy and preparing for germination; cell wall weakening and remodeling processes were activated to provide protection for cotyledon protrusion; in addition, multiple antioxidant systems were activated to alleviate oxidative stress generated during the germination process; ERF transcription factor has the highest number in both stages suggested an active role in eelgrass seed germination.
CONCLUSIONS: In summary, for the first time, the present study investigated the mechanisms by which eelgrass seed germination was stimulated by low salinity and analyzed the transcriptomic and proteomic features during eelgrass seed germination comprehensively. The results of the present study enhanced our understanding of seagrass seed germination, especially the molecular ecology of seagrass seeds.
摘要：
背景：ZosteramarinaL.，或者鳗草,是整个北半球温带最广泛的海草物种。与陆地植物的干燥种子不同，鳗草种子必须在水中生存，盐度是影响鳗草种子萌发的关键因素。在本研究中,结合转录组和蛋白质组分析，研究低盐度刺激鳗草种子萌发的机制，除了发芽下关键代谢途径的动力学。
结果：根据结果，低盐度刺激Ca2+信号和磷脂酰肌醇信号的激活，并通过MAPK转导级联进一步启动了各种与发芽相关的生理过程。淀粉,脂质，和贮藏蛋白被积极动员，为发芽提供能量和物质基础；脱落酸的合成和信号转导被抑制，赤霉素的合成和信号转导被激活，削弱种子休眠，准备发芽；激活细胞壁弱化和重塑过程，为子叶突出提供保护；此外，多个抗氧化系统被激活以减轻发芽过程中产生的氧化应激；ERF转录因子在两个阶段中的数量最高，表明在鳗草种子萌发中具有积极作用。
结论：总之，第一次,本研究探讨了低盐度刺激鳗草种子萌发的机制，并全面分析了鳗草种子萌发过程中的转录组学和蛋白质组学特征。本研究的结果增强了我们对海草种子萌发的理解，特别是海草种子的分子生态学。
结果：根据结果，低盐度刺激Ca2+信号和磷脂酰肌醇信号的激活，并通过MAPK转导级联进一步启动了各种与发芽相关的生理过程。淀粉,脂质，和贮藏蛋白被积极动员，为发芽提供能量和物质基础；脱落酸的合成和信号转导被抑制，赤霉素的合成和信号转导被激活，削弱种子休眠，准备发芽；激活细胞壁弱化和重塑过程，为子叶突出提供保护；此外，多个抗氧化系统被激活以减轻发芽过程中产生的氧化应激；ERF转录因子在两个阶段中的数量最高，表明在鳗草种子萌发中具有积极作用。
结论：总之，第一次,本研究探讨了低盐度刺激鳗草种子萌发的机制，并全面分析了鳗草种子萌发过程中的转录组学和蛋白质组学特征。本研究的结果增强了我们对海草种子萌发的理解，特别是海草种子的分子生态学。
