PDF(Pubmed)
Abstract：
UNASSIGNED: Due to the disadvantages of manual disinfection of patient rooms, mobile disinfection robots using ultraviolet C (UV-C) radiation are increasingly being used. Assessing their in situ effectiveness remains challenging.
UNASSIGNED: This study describes a new method to prove adequate in situ disinfection (≥5-log reduction in bacterial load), and uses this method to assess the efficacy of a mobile disinfection robot using UV-C radiation.
UNASSIGNED: Agar plates serving as proxies for smooth surfaces in patient rooms were inoculated with bacterial suspension and placed on various surfaces in a patient room. After irradiation by an automated mobile UV-C robot, reduction in colony growth was determined by comparing the irradiated plates to a reference series of non-irradiated plates, enabling the evaluation of whether an adequate reduction in colony-forming units (CFU\'s) of ≥5-log was reached on these irradiated surfaces.
UNASSIGNED: The new technique described here proved a successful method for demonstrating an in situ ≥5-log reduction in CFU\'s for five different bacterial pathogens. Of the 32 plates placed on UV-accessible surfaces, 31 showed an adequate reduction in CFU\'s of ≥5-log. One plate could not be assessed.
UNASSIGNED: Inoculated agar plates placed in patient rooms before irradiation and subsequently compared to a reference series can be used to assess in situ efficacy of mobile disinfection robots using UV-C radiation. Our findings support the idea that UV-C robots, used adjunctively to conventional manual washing and disinfection, may achieve adequate bacterial load reduction on UV-accessible smooth surfaces in patient rooms for a selected subset of pathogens.
UNASSIGNED: This study describes a new method to prove adequate in situ disinfection (≥5-log reduction in bacterial load), and uses this method to assess the efficacy of a mobile disinfection robot using UV-C radiation.
UNASSIGNED: Agar plates serving as proxies for smooth surfaces in patient rooms were inoculated with bacterial suspension and placed on various surfaces in a patient room. After irradiation by an automated mobile UV-C robot, reduction in colony growth was determined by comparing the irradiated plates to a reference series of non-irradiated plates, enabling the evaluation of whether an adequate reduction in colony-forming units (CFU\'s) of ≥5-log was reached on these irradiated surfaces.
UNASSIGNED: The new technique described here proved a successful method for demonstrating an in situ ≥5-log reduction in CFU\'s for five different bacterial pathogens. Of the 32 plates placed on UV-accessible surfaces, 31 showed an adequate reduction in CFU\'s of ≥5-log. One plate could not be assessed.
UNASSIGNED: Inoculated agar plates placed in patient rooms before irradiation and subsequently compared to a reference series can be used to assess in situ efficacy of mobile disinfection robots using UV-C radiation. Our findings support the idea that UV-C robots, used adjunctively to conventional manual washing and disinfection, may achieve adequate bacterial load reduction on UV-accessible smooth surfaces in patient rooms for a selected subset of pathogens.
摘要：
■由于手动消毒病房的缺点，越来越多地使用使用紫外线C（UV-C）辐射的移动式消毒机器人。评估其原位有效性仍然具有挑战性。
■这项研究描述了一种新的方法来证明适当的原位消毒（细菌负荷减少≥5-log），并使用该方法评估使用UV-C辐射的移动消毒机器人的功效。
■用细菌悬浮液接种用作病房中光滑表面的代理的琼脂板,并放置在病房中的各种表面上。在自动移动UV-C机器人照射后，菌落生长的减少是通过将辐照的平板与未辐照的参考系列平板进行比较来确定的，能够评估在这些辐照表面上是否达到菌落形成单位(CFU)≥5-log的充分减少。
■此处描述的新技术证明了一种成功的方法，用于证明五种不同细菌病原体的CFU的原位≥5-log减少。在放置在紫外线可接触表面上的32个板中，31显示CFU的足够降低≥5-log。一个板块无法评估。
■在照射之前放置在病房中并随后与参考系列进行比较的接种琼脂板可用于评估使用UV-C辐射的移动消毒机器人的原位功效。我们的发现支持UV-C机器人，用于辅助常规手动洗涤和消毒，对于选定的病原体子集，可以在病房中的紫外线可接触的光滑表面上实现足够的细菌负荷减少。
■这项研究描述了一种新的方法来证明适当的原位消毒（细菌负荷减少≥5-log），并使用该方法评估使用UV-C辐射的移动消毒机器人的功效。
■用细菌悬浮液接种用作病房中光滑表面的代理的琼脂板,并放置在病房中的各种表面上。在自动移动UV-C机器人照射后，菌落生长的减少是通过将辐照的平板与未辐照的参考系列平板进行比较来确定的，能够评估在这些辐照表面上是否达到菌落形成单位(CFU)≥5-log的充分减少。
■此处描述的新技术证明了一种成功的方法，用于证明五种不同细菌病原体的CFU的原位≥5-log减少。在放置在紫外线可接触表面上的32个板中，31显示CFU的足够降低≥5-log。一个板块无法评估。
■在照射之前放置在病房中并随后与参考系列进行比较的接种琼脂板可用于评估使用UV-C辐射的移动消毒机器人的原位功效。我们的发现支持UV-C机器人，用于辅助常规手动洗涤和消毒，对于选定的病原体子集，可以在病房中的紫外线可接触的光滑表面上实现足够的细菌负荷减少。
