Abstract：
BACKGROUND: The reactivity of blood with non-endothelial surface is a challenge for long-term Ventricular Assist Devices development, usually made with pure titanium, which despite of being inert, low density and high mechanical resistance it does not avoid the thrombogenic responses. Here we tested a modification on the titanium surface with Laser Induced Periodic Surface Structures followed by Diamond Like Carbon (DLC) coating in different thicknesses to customize the wettability profile by changing the surface energy of the titanium.
METHODS: Four different surfaces were proposed: (1) Pure Titanium as Reference Material (RM), (2) Textured as Test Sample (TS), (3) Textured with DLC 0.3μm as (TSA) and (4) Textured with 2.4μm DLC as (TSB). A single implant was positioned in the abdominal aorta of Wistar rats and the effects of hemodynamic interaction were evaluated without anticoagulant drugs.
RESULTS: After twelve weeks, the implants were extracted and subjected to qualitative analysis by Scanning Electron Microscopy under low vacuum and X-ray Energy Dispersion. The regions that remained in contact with the wall of the aorta showed encapsulation of the endothelial tissue. TSB implants, although superhydrophilic, have proven that the DLC coating inhibits the adhesion of biological material, prevents abrasive wear and delamination, as observed in the TS and TSA implants. Pseudo- neointimal layers were heterogeneously identified in higher concentration on Test Surfaces.
METHODS: Four different surfaces were proposed: (1) Pure Titanium as Reference Material (RM), (2) Textured as Test Sample (TS), (3) Textured with DLC 0.3μm as (TSA) and (4) Textured with 2.4μm DLC as (TSB). A single implant was positioned in the abdominal aorta of Wistar rats and the effects of hemodynamic interaction were evaluated without anticoagulant drugs.
RESULTS: After twelve weeks, the implants were extracted and subjected to qualitative analysis by Scanning Electron Microscopy under low vacuum and X-ray Energy Dispersion. The regions that remained in contact with the wall of the aorta showed encapsulation of the endothelial tissue. TSB implants, although superhydrophilic, have proven that the DLC coating inhibits the adhesion of biological material, prevents abrasive wear and delamination, as observed in the TS and TSA implants. Pseudo- neointimal layers were heterogeneously identified in higher concentration on Test Surfaces.
摘要：
背景：血液与非内皮表面的反应性是长期心室辅助装置开发的挑战，通常用纯钛制成，尽管是惰性的，低密度和高机械阻力它并不能避免血栓形成反应。在这里，我们测试了用激光诱导的周期性表面结构对钛表面的改性，然后是不同厚度的类金刚石碳（DLC）涂层，以通过改变钛的表面能来定制润湿性轮廓。
方法：提出了四种不同的表面：（1）纯钛作为参考材料（RM），（2）纹理作为测试样品（TS），(3)用DLC(0.3μm)织构化为(TSA)和(4)用2.4μmDLC(TSB)织构化。将单个植入物放置在Wistar大鼠的腹主动脉中，并在没有抗凝药物的情况下评估血液动力学相互作用的影响。
结果：12周后，提取植入物，并在低真空和X射线能量分散下通过扫描电子显微镜进行定性分析。保持与主动脉壁接触的区域显示内皮组织的包封。TSB植入物,虽然超亲水，已经证明DLC涂层抑制了生物材料的粘附，防止磨料磨损和分层，如在TS和TSA植入物中观察到的。在测试表面上以较高浓度不均匀地鉴定了假内膜层。
方法：提出了四种不同的表面：（1）纯钛作为参考材料（RM），（2）纹理作为测试样品（TS），(3)用DLC(0.3μm)织构化为(TSA)和(4)用2.4μmDLC(TSB)织构化。将单个植入物放置在Wistar大鼠的腹主动脉中，并在没有抗凝药物的情况下评估血液动力学相互作用的影响。
结果：12周后，提取植入物，并在低真空和X射线能量分散下通过扫描电子显微镜进行定性分析。保持与主动脉壁接触的区域显示内皮组织的包封。TSB植入物,虽然超亲水，已经证明DLC涂层抑制了生物材料的粘附，防止磨料磨损和分层，如在TS和TSA植入物中观察到的。在测试表面上以较高浓度不均匀地鉴定了假内膜层。
