PDF(Pubmed)
Abstract：
Cattle cryptosporidiosis is noted worldwide with varied frequency of infection prevalence depending on geographical, environmental and husbandry factors. In this study, the prevalence of Cryptosporidium infections in cattle was determined on the basis of molecular results obtained by testing 1601 faecal samples collected from calves up to 4 months of age housed in all Polish provinces from 2014 to 2018. Detection and identification of Cryptosporidium species was performed at the 18 small subunit ribosomal RNA (18S rRNA) locus by conducting PCR-RFLP analysis of the amplified DNA fragments. The prevalence of Cryptosporidium infections in the cattle population was 45.3% (CI 95%: 42.8-47.7; 725/1601). The infected animals were housed on 233/267 (87.3%) of monitored farms with regional prevalence ranging from 27.8 to 62%. The restriction pattern of 18S rRNA amplicons for positive samples was characteristic of C. parvum, C. bovis, C. ryanae, C. andersoni, and unexpectedly also of C. baileyi and C. suis. Infections of C. bovis and C. ryanae prevailed in the studied cattle population relegating C. parvum to third in prevalence. Likewise, mixed infections caused by C. bovis and C. ryanae as well as C. parvum and C. bovis were observed. A relationship between the infecting parasite species and animal breed was found. For instance, C. parvum prevailed in Black and White lowland breed, C. ryanae in Limousine cattle and C. andersoni in dairy animals of mixed dairy breeds. Furthermore, differences in prevalence of particular parasite species between cattle breeds were also shown.
摘要：
牛隐孢子虫病在全世界范围内都有不同的感染率，具体取决于地理，环境和畜牧业因素。在这项研究中,牛中隐孢子虫感染的流行率是根据2014年至2018年期间从波兰所有省份4月龄以下小牛收集的1601份粪便样本进行检测获得的分子结果确定的.通过对扩增的DNA片段进行PCR-RFLP分析，在18小亚基核糖体RNA（18SrRNA）基因座上进行了隐孢子虫物种的检测和鉴定。牛群中隐孢子虫感染的患病率为45.3％（CI95％：42.8-47.7；725/1601）。受感染的动物饲养在233/267（87.3％）的受监测农场中，区域患病率为27.8％至62％。阳性样品的18SrRNA扩增子的限制性图谱是C.parvum的特征，C.牛，C.ryanae,C.Andersoni,出乎意料的是C.baileyi和C.suis。在所研究的牛种群中，牛C.cavis和C.ryanae的感染占主导地位，将C.parvum的患病率降至第三位。同样，观察到由牛梭菌和Ryanae以及C.parvum和C.bovis引起的混合感染。发现了感染寄生虫物种与动物品种之间的关系。例如,C.parvum在黑白低地品种中盛行，豪华轿车牛的ryanae和混合乳品品种的奶牛的C.andersoni。此外，还显示了牛品种之间特定寄生虫物种的患病率差异。
