BACKGROUND: NSPT is the gold-standard treatment for periodontal pockets < 6 mm in depth, however, successful outcomes are not always guaranteed due to several factors. Periodontitis-associated tissue destruction is evidenced by the increased level of soluble E-cadherin and reduced antioxidants in oral fluids which could be used as predictors for success/failure of NSPT.
METHODS: Patients with periodontitis (n = 24) were included in this clinical trial and full-mouth periodontal charting was recorded for each patient. GCF samples from periodontal pockets with probing pocket depth (PPD) 4-6 mm from the interproximal surfaces of anterior and premolar teeth were obtained. These sites subsequently received NSPT and were clinically re-evaluated after 1 and 3 months. Levels of GCF E-cadherin and TAC levels were assayed using ELISA.
RESULTS: All clinical periodontal parameters were significantly improved 3 months after completion of NSPT. These outcomes were associated with a significant decrease in E-cadherin levels and GCF volume, while TAC levels were significantly increased in samples obtained in follow-up appointments. Binary regression model analysis showed that PPD, GCF volume, E-cadherin, and TAC levels could significantly (p < .05) predict the outcomes of NSPT. The cut-off points for PPD, GCF volume, E-cadherin and TAC were 5 mm, 4 × 10-3, 1267.97 pg/mL and 0.09 μmol/g, respectively.
CONCLUSIONS: NSPT improved clinical parameters along with increased antioxidants capacity and epithelial pocket lining integrity. Discrimination of favorable/unfavorable responsiveness of periodontally diseased sites to NSPT could be possible by using GCF volume, PPD, E-cadherin and TAC level assessments.
背景:NSPT是治疗深度<6mm牙周袋的金标准,然而,由于几个因素,成功的结果并不总是得到保证。口腔液中可溶性E-钙黏着蛋白水平的增加和抗氧化剂的减少证明了牙周炎相关的组织破坏,这可以用作NSPT成功/失败的预测因子。
方法:这项临床试验包括牙周炎患者(n=24),并记录每位患者的全口牙周图。从牙周袋中获得GCF样本,其探测袋深度(PPD)距前磨牙和前磨牙的邻间表面4-6mm。这些部位随后接受NSPT,并在1个月和3个月后进行临床重新评估。使用ELISA测定GCF-E-钙黏着蛋白的水平和TAC水平。
结果:完成NSPT后3个月,所有临床牙周参数均有明显改善。这些结果与E-cadherin水平和GCF体积的显着下降有关,而在随访预约中获得的样本中TAC水平显著升高。二元回归模型分析表明,PPD,GCF卷,E-cadherin,TAC水平可以显着(p<0.05)预测NSPT的结果。PPD的截止点,GCF卷,E-cadherin和TAC为5mm,4×10-3,1267.97pg/mL和0.09μmol/g,分别。
结论:NSPT改善了临床参数,同时增加了抗氧化剂的能力和上皮袋的完整性。通过使用GCF体积,可以区分牙周病变部位对NSPT的有利/不利反应,PPD,E-cadherin和TAC水平评估。