Abstract：
The growth and development of early mammalian embryos mainly take place in the fallopian tube, which not only provides nutrients for embryonic growth and development but also offers suitable mechanical conditions. The embryo culture system established in assisted reproductive technology mainly simulates the environment in which oocytes and embryos grow and develop in vivo. However, current in vitro embryo culture is mainly static and cannot completely mimic the mechanical environment in which embryos grow and develop in vivo. Therefore, to more accurately simulate the mechanical environment of embryos in the fallopian tube, we have developed a dynamic culture device to investigate the effects of mechanical stimulation on the in vitro maturation of immature oocytes and their parthenogenetic developmental potential. Immature mice oocytes were subjected to in vitro maturation by static culture and vibration (3 Hz, 6 Hz) with tilting for 15∼16 hours. The maturation of oocytes was observed after the culture period. The mature oocytes were activated by parthenogenesis and the rate of embryo compaction and formation of parthenogenetic blastocysts was analyzed. The results showed that using 3 Hz vibration and tilting can significantly improve the parthenogenetic development potential of immature mice oocytes.
摘要：
哺乳动物早期胚胎的生长发育主要发生在输卵管,它不仅为胚胎生长和发育提供营养，还提供合适的机械条件。辅助生殖技术中建立的胚胎培养系统主要模拟卵母细胞和胚胎在体内生长发育的环境。然而,目前的体外胚胎培养主要是静态的，不能完全模拟胚胎在体内生长和发育的机械环境。因此,为了更准确地模拟输卵管中胚胎的机械环境，我们开发了一种动态培养装置，以研究机械刺激对未成熟卵母细胞体外成熟及其孤雌生殖发育潜力的影响。未成熟小鼠卵母细胞通过静态培养和振动（3Hz，6赫兹)倾斜15~16小时。培养期后观察卵母细胞的成熟。通过孤雌生殖激活成熟卵母细胞，并分析胚胎压实率和孤雌生殖囊胚形成率。结果表明，使用3Hz振动和倾斜可以显着提高未成熟小鼠卵母细胞的孤雌生殖发育潜能。
