Abstract：
The physiological response to feeding is important for production aspects that include feed utilization and growth, and the responses require the action of numerous secretory factors. However, as an important aquaculture animal, the secretory response of Pacific White Shrimp (Litopenaeus vannamei) after feeding has not been comprehensively characterized. In this study, transcriptome analysis showed that 3172 differentially expressed genes were involved in the post-feeding response, including 289 new genes not annotated in the L. vannamei reference genome. Subsequently, 715 differentially expressed secretory reference genes and 18 new differentially expressed secretory genes were obtained through the identification of signal peptides in secreted proteins. Functional classification revealed that differentially expressed secretory genes were enriched in pathways pertaining to lipid metabolism (20 genes), carbohydrate metabolism (21 genes), glycan biosynthesis and metabolism (27 genes), digestive system (40 genes), and transport and metabolism (43 genes). The 14 pathways most enriched by differentially expressed secretory genes involved 83 genes, 71 of which encoded enzymes involved in food digestion and metabolism. Specific enzymes such as lipase 3-like and NPC intracellular cholesterol transporter 1-like in lipid metabolism, alpha-amylase-like and glucosylceramidase-like in carbohydrate metabolism, and cysteine proteinase 4-like and trypsin-1-like in the digestive system were found to be differentially expressed. Furthermore, we discovered a new gene, MSTRG.2504, that participates in the digestive system and carbohydrate metabolism. The study provides valuable insights into the secretory response (especially metabolism-related enzymes) to feeding in L. vannamei, uncovering the significant roles of both known and new genes. Furthermore, this study will improve our understanding of the feeding physiology of L. vannamei and provide a reference basis for further feeding endocrine research in the future.
摘要：
对饲料的生理反应对于包括饲料利用和生长在内的生产方面很重要。这些反应需要许多分泌因子的作用。然而,作为一种重要的水产动物，饲养后太平洋白虾（凡纳滨对虾）的分泌反应尚未得到全面表征。在这项研究中,转录组分析表明，3172个差异表达基因参与了喂养后的反应，包括289个未在南美白对虾参考基因组中注释的新基因。随后，通过对分泌蛋白中信号肽的鉴定,获得了715个差异表达的分泌内参基因和18个新的差异表达的分泌基因。功能分类显示，差异表达的分泌基因富集在与脂质代谢有关的途径中（20个基因），碳水化合物代谢（21个基因），聚糖生物合成和代谢（27个基因），消化系统（40个基因），以及运输和代谢（43个基因）。差异表达分泌基因最丰富的14条途径涉及83个基因，其中71种编码参与食物消化和代谢的酶。脂质代谢中的特定酶，例如脂肪酶3样和NPC细胞内胆固醇转运蛋白1样，碳水化合物代谢中的α-淀粉酶样和葡糖神经酰胺酶样，发现消化系统中的半胱氨酸蛋白酶4样和胰蛋白酶1样差异表达。此外，我们发现了一个新的基因,MSTRG.2504，参与消化系统和碳水化合物代谢。该研究为凡纳滨对虾的分泌反应（尤其是代谢相关的酶）提供了有价值的见解。揭示已知基因和新基因的重要作用。此外，本研究将提高我们对南美白对虾饲养生理的认识,为今后进一步的饲养内分泌研究提供参考依据。
