Abstract：
A new electrochemical biosensor based on the sequence of chromosome Y (SRY) has been introduced to determine the gender of the fetus. At first, the DNA probe was designed based on the SRY gene sequence on chromosome Y. Then, a suitable functional group was added to the DNA probe, and it has been immobilized on the surface of the electrode modified with a nanocomposite containing Cu(OH)2 @N-C n-boxes. This substrate causes more DNA probes to connect to the electrode surface by increasing the effective surface area. The presence of the SRY sequence in the DNA sample extracted from blood was detected by the electrochemical signal of the bio-sensor. After optimizing the parameters, the fabricated genosensor showed linear responses in the two concentration ranges containing 0.5 fM to 50 pM and 50 pM to 500 nM. The limit of detection (LOD) for the proposed method was 0.16 fM. The proposed genosensor has been successfully used to determine the gender of the fetus using cell-free fetal DNA (cffDNA) in the blood plasma of several pregnant mothers. This method has advantages such as being simple, portable, accurate, and non-invasive for early determination of the gender of the fetus and early diagnosis of X-linked genetic disorders.
摘要：
已引入基于Y染色体（SRY）序列的新型电化学生物传感器来确定胎儿的性别。起初,根据染色体Y上的SRY基因序列设计DNA探针。然后，一个合适的官能团被添加到DNA探针，并将其固定在用含Cu（OH）2@N-Cn盒的纳米复合材料修饰的电极表面上。该底物通过增加有效表面积使更多的DNA探针连接到电极表面。通过生物传感器的电化学信号检测从血液中提取的DNA样品中SRY序列的存在。优化参数后，制造的基因传感器在包含0.5fM至50pM和50pM至500nM的两个浓度范围内显示出线性响应。该方法的检测限(LOD)为0.16fM。拟议的基因传感器已成功用于使用几位孕妇血浆中的无细胞胎儿DNA（cffDNA）确定胎儿的性别。该方法具有简单,便携式，准确,和非侵入性的早期确定胎儿的性别和X连锁遗传疾病的早期诊断。
