PDF(Pubmed)
Abstract：
Kudzu (Pueraria montana var. lobata), a plant native to Southeastern Asia, has become a major noxious weed covering millions of hectares in the Southern United States. A kudzu patch displaying virus-like symptoms located in Ackerman, northeastern Mississippi (MS), was used as a source for virus isolation and characterization involving mechanical and vector transmission, ultrastructural observation, surveys, Sanger and high-throughput genome sequencing, and sequence analyses. The results revealed the presence of a new potyvirus in infected kudzu, closely related to wisteria vein mosaic virus (WVMV) and provisionally named kudzu chlorotic ring blotch virus (KudCRBV). Genome features and pairwise comparison with six WVMV genomes currently available in GenBank and three additional isolates from MS sequenced in this work suggest that KudCRBV is likely a member of a new species in the genus Potyvirus. Furthermore, under experimental conditions, KudCRBV was successfully transmitted by cotton and potato aphids and mechanically to soybean and beans. A state-wide survey revealed several kudzu patches infected by the virus in northern MS.
摘要：
葛藤（蒙大拿州葛根var。lobata),一种原产于东南亚的植物，已经成为美国南部数百万公顷的主要有害杂草。阿克曼有一个显示病毒样症状的葛根补丁，密西西比州东北部（MS），被用作涉及机械和媒介传播的病毒分离和表征的来源，超微结构观察，调查,Sanger和高通量基因组测序，和序列分析。结果显示，在受感染的葛藤中存在一种新的马铃薯病毒，与紫藤脉花叶病毒（WVMV）和暂时命名为葛根褪绿环斑病病毒（KudCRBV）密切相关。基因组特征以及与GenBank中目前可用的六个WVMV基因组和这项工作中测序的来自MS的三个其他分离株的成对比较表明，KudCRBV可能是Potyvirus属新物种的成员。此外，在实验条件下，KudCRBV通过棉花和马铃薯蚜虫成功传播，并机械传播到大豆和豆类。一项全州范围的调查显示，在MS北部有几个被病毒感染的葛根斑块。
