PDF(Pubmed)
Abstract：
In this overview, the latest achievements in dietary origins, absorption mechanism, bioavailability assay, health advantages, cutting-edge encapsulation techniques, fortification approaches, and innovative highly sensitive sensor-based detection methods of vitamin B12 (VB12) were addressed. The cobalt-centered vitamin B is mainly found in animal products, posing challenges for strict vegetarians and vegans. Its bioavailability is highly influenced by intrinsic factor, absorption in the ileum, and liver reabsorption. VB12 mainly contributes to blood cell synthesis, cognitive function, and cardiovascular health, and potentially reduces anemia and optic neuropathy. Microencapsulation techniques improve the stability and controlled release of VB12. Co-microencapsulation of VB12 with other vitamins and bioactive compounds enhances bioavailability and controlled release, providing versatile initiatives for improving bio-functionality. Nanotechnology, including nanovesicles, nanoemulsions, and nanoparticles can enhance the delivery, stability, and bioavailability of VB12 in diverse applications, ranging from antimicrobial agents to skincare and oral insulin delivery. Staple food fortification with encapsulated and free VB12 emerges as a prominent strategy to combat deficiency and promote nutritional value. Biosensing technologies, such as electrochemical and optical biosensors, offer rapid, portable, and sensitive VB12 assessment. Carbon dot-based fluorescent nanosensors, nanocluster-based fluorescent probes, and electrochemical sensors show promise for precise detection, especially in pharmaceutical and biomedical applications.
摘要：
在本概述中,饮食起源的最新成就，吸收机制，生物利用度测定，健康优势,尖端封装技术，设防方法，提出了基于高灵敏度传感器的维生素B12（VB12）检测方法。以钴为中心的维生素B主要存在于动物产品中，对严格的素食主义者和素食主义者构成挑战。它的生物利用度受到内在因素的高度影响，在回肠吸收，和肝脏的重吸收。VB12主要有助于血细胞合成,认知功能,和心血管健康，并有可能减少贫血和视神经病变。微囊化技巧进步了VB12的稳固性和控释。VB12与其他维生素和生物活性化合物的共微囊化增强了生物利用度和控制释放，提供多功能的举措，以提高生物功能。纳米技术，包括纳米囊泡，纳米乳液，纳米颗粒可以增强递送，稳定性,以及VB12在各种应用中的生物利用度，从抗菌剂到护肤和口服胰岛素。具有包封和游离VB12的主食强化作为对抗缺乏和提高营养价值的突出策略。生物传感技术,如电化学和光学生物传感器，提供快速，便携式，和敏感的VB12评估。基于碳点的荧光纳米传感器，基于纳米团簇的荧光探针，和电化学传感器显示出精确检测的希望，特别是在制药和生物医学应用中。
