PDF(Pubmed)
Abstract：
Trichomonas gallinae (T. gallinae) has a great influence on the pigeon industry. Pigeons display different resistance abilities to T. gallinae, so the study of the molecular mechanism of resistance is necessary in breeding disease resistant lines. MiRNA plays important roles in the immune response, but there are still no reports of miRNA regulating trichomonosis resistance. We used small RNA sequencing technology to characterize miRNA profiles in different groups. T. gallinae was nasally inoculated in one day old squabs, and according to the infection status, the groups were divided into control (C), susceptible (S) and tolerant (T) groups. We identified 2429 miRNAs in total, including 1162 known miRNAs and 1267 new miRNAs. In a comparison among the C, S and T groups, the target genes of differentially expressed miRNAs were analyzed via GO and KEGG annotation. The results showed that the target genes were enriched in immune-response-related pathways. This indicated that the differentially expressed miRNAs had a critical influence on T. gallinae infection. Novel_miR_741, which could inhibit the expression of PRKCQ, was down-regulated in the T group compared to the C group. It was proven that a decreased novel_miR_741 expression would increase the expression of PRKCQ and increase the immune response. This study brings new insights into understanding the mechanism of trichomonosis resistance.
摘要：
鸡毛滴虫(T.gallinae）对鸽子产业有很大的影响。鸽子对鸡巴表现出不同的抗性，因此研究抗病分子机制是选育抗病系的必要条件。miRNA在免疫应答中起重要作用,但目前尚无miRNA调控滴虫病耐药的报道。我们使用小RNA测序技术来表征不同组中的miRNA谱。胆虫在一天大的乳鼠中进行了鼻腔接种，根据感染状况,将各组分为对照组（C），易感（S）和耐受（T）组。我们总共鉴定了2429个miRNAs，包括1162个已知的miRNA和1267个新的miRNA。在C之间的比较中，S和T组，通过GO和KEGG注释分析差异表达miRNAs的靶基因。结果表明，靶基因在免疫应答相关途径中富集。这表明差异表达的miRNA对鸡木霉感染具有关键影响。能抑制PRKCQ表达的新型miR_741,与C组相比，T组下调。结果表明，降低new_miR_741的表达会增加PRKCQ的表达并增加免疫应答。这项研究为理解滴虫病耐药机制带来了新的见解。
