PDF(Pubmed)
Abstract：
Health-oriented preferences, a demand for innovative food concepts, and technological advances have greatly influenced changes in the food industry and led to remarkable development of the functional food market. Incorporating herbal extracts as a rich source of bioactive compounds (BC) could be an effective solution to meet the high demand of consumers in terms of expanding the high-quality range of functional foods. The aim of this study is the valorization of the bioactive potential of T. montanum L., an understudied Mediterranean plant species, and the in-depth elucidation of a polyphenolic profile with a UHPLC-HR MS/MS and NMR analysis. The total phenolic content (TPC) and antioxidant capacity (AC) were determined on heat-assisted (HAE), microwave-assisted (MAE) and subcritical water (SWE) extracts. In terms of antioxidant capacity, SWE extracts showed the most notable potential (ABTS: 0.402-0.547 mmol eq Trolox g-1 dw, DPPH: 0.336-0.427 mmol eq Trolox g-1 dw). 12 phenolic compounds were identified in the samples of T. montanum from six microlocations in Croatia, including nine phenylethanoid glycosides (PGs) with total yields of 30.36-68.06 mg g-1 dw and 25.88-58.88 mg g-1 dw in HAE and MAE extracts, respectively. Echinacoside, teupolioside, stachysoside A, and poliumoside were the most abundant compounds HAE and MAE extracts, making T. montanum an emerging source of PGs.
摘要：
以健康为导向的偏好，对创新食品概念的需求，和技术进步极大地影响了食品工业的变化，并导致了功能食品市场的显着发展。将草药提取物作为生物活性化合物（BC）的丰富来源可能是满足消费者在扩大功能食品的高质量范围方面的高需求的有效解决方案。这项研究的目的是提高T.montanumL.的生物活性潜力，一种研究不足的地中海植物，并通过UHPLC-HRMS/MS和NMR分析深入阐明了多酚谱。在热辅助（HAE）上测定总酚含量（TPC）和抗氧化能力（AC），微波辅助（MAE）和亚临界水（SWE）提取物。在抗氧化能力方面，SWE提取物显示出最显着的潜力（ABTS：0.402-0.547mmoleqTroloxg-1dw，DPPH:0.336-0.427mmol当量Troloxg-1dw)。在克罗地亚六个微地点的T.montanum样品中鉴定出12种酚类化合物，包括九种苯乙醇苷（PG），在HAE和MAE提取物中的总产量为30.36-68.06mgg-1dw和25.88-58.88mgg-1dw，分别。松果苷,teupolioside,水苏苷A,和脊髓灰质炎苷是最丰富的化合物HAE和MAE提取物，使T.montanum成为新兴的PG来源。
