Abstract：
The environmental risks of trifloxystrobin (TR) have drawn attention because of its multiplex toxicity on aquatic organisms, but few studies have paid close attention to its chronic toxicity at environmental concentrations. In present study, histopathology, metabolomics and transcriptomics were comprehensively performed to investigate the toxic effects and biological responses on adult zebrafish after exposure to 0.1, 1 and 10 μg/L TR for 21 d. Results demonstrated long-term exposure of TR affected zebrafish liver, ovary and heart development. Metabolomics revealed 0.1, 1 and 10 μg/L TR simultaneously decreased the carbohydrates enriched in glucose metabolism and ABC transporters pathways, such as glycogen, lactose, lactulose, maltose, maltotriose, d-trehalose, while 1 μg/L and 10 μg/L TR significantly increased many metabolites related to glycerophospholipid and sphingolipid metabolism in zebrafish liver. Transcriptomics showed TR activated the transcription of the Abcb4, Abcb5 and Abcb11 involved in ABC transporters, Pck1, Pfk, Hk, Gyg1a and Pygma related to glucose metabolism, as well as the Lpcat1, Lpcat4, Gpat2, Cers and Sgms in glycerophospholipid and sphingolipid metabolism. Results further demonstrated high concentration of TR strongly affected the DNA repair system, while low dose of TR caused pronounced effects on cardiomyocytes and oocyte regulation pathways at transcriptional levels. The results indicated the abnormal liver, gonad and heart development caused by TR might be ascribed to the disturbance of carbohydrates and lipid metabolism mediating by the Abcb4, Abcb5 and Abcb11 ABC transporters, and long-term exposure of environmental concentration of TR was sufficient to affect zebrafish normal metabolism and development.
摘要：
由于其对水生生物的多重毒性，三氟霉素（TR）的环境风险引起了人们的关注。但是很少有研究密切关注其在环境浓度下的慢性毒性。在目前的研究中,组织病理学,代谢组学和转录组学进行了综合研究，以研究暴露于0.1，1和10μg/LTR21d后对成年斑马鱼的毒性作用和生物学反应。卵巢和心脏发育。代谢组学显示0.1、1和10μg/LTR同时降低了富含葡萄糖代谢和ABC转运蛋白途径的碳水化合物，比如糖原，乳糖,乳果糖,麦芽糖,麦芽三糖,d-海藻糖,而1μg/L和10μg/LTR显著增加斑马鱼肝脏中与甘油磷脂和鞘脂代谢相关的许多代谢产物。转录组学显示TR激活了ABC转运蛋白Abcb4，Abcb5和Abcb11的转录，pck1,Pfk,香港,Gyg1a和Pygma与葡萄糖代谢有关，以及甘油磷脂和鞘脂代谢中的Lpcat1，Lpcat4，Gpat2，Cers和Sgms。结果进一步证明高浓度的TR强烈影响DNA修复系统，而低剂量的TR在转录水平上对心肌细胞和卵母细胞调节途径产生明显影响。结果显示肝脏异常，TR引起的性腺和心脏发育可能归因于Abcb4，Abcb5和Abcb11ABC转运蛋白介导的碳水化合物和脂质代谢紊乱，且长期暴露于环境浓度的TR足以影响斑马鱼的正常代谢和发育。
