Abstract：
An obligate anaerobic, Gram-negative, rod-shaped and non-spore-forming bacterium, designated as strain GYB001T, was isolated from the blood of a patient with a sigmoid colon perforation. Taxonomic characterization of the novel isolate was performed using a polyphasic approach. A phylogenetic analysis based on 16S rRNA gene and whole genome sequences revealed that GYB001T represented a member of the genus Parabacteroides, in the family Tannerellaceae. The closest species, based on 16S rRNA sequence, was Parabacteroides gordonii DSM 23371T with 97.4 % similarity. Average nucleotide identity and digital DNA-DNA hybridization values between strain GYB001T and P. gordonii DSM 23371T were 86.7 and 28.7% and between GYB001T and Parabacteroides faecis JCM 18682T were 86.6 and 27.7 %, respectively. The genome was 6.57 Mbp long with 43.3 mol% G+C content. Colonies on Brucella blood agar (BBA) were circular, convex, smooth, grey and small in size. Growth was observed on trypticase soy agar (TSA), TSA +5 % sheep blood and Euglena gracilis agar. Growth occurred at 18-42 °C on BBA in the presence of 0-3 % NaCl (w/v) and at pH 6.0-8.5. The major polar lipids were phosphatidylethanolamine and phospholipids. The major fatty acids in strain GYB001T were anteiso-C15 : 0 and iso-C17 : 0 3-OH, and the predominant respiratory quinones were menaquinone-10 (MK-10) and MK-9. The cell wall contained meso-diaminopimelic acid. Considering these phenotypic features and comparative genome analyses, we propose strain GYB001T as the type strain of Parabacteroides leei sp. nov. (=KCTC 25738T=KBN12P06525T=LMG 32797T).
摘要：
专性厌氧菌,革兰氏阴性,杆状和非孢子形成细菌，指定为菌株GYB001T，从乙状结肠穿孔患者的血液中分离出来。使用多相方法对新型分离物进行了分类学表征。基于16SrRNA基因和全基因组序列的系统发育分析表明，GYB001T代表副杆菌属的成员，在制革兰科。最接近的物种,基于16SrRNA序列，格氏副杆菌属DSM23371T，相似性为97.4%。菌株GYB001T和格氏疟原虫DSM23371T之间的平均核苷酸同一性和数字DNA-DNA杂交值分别为86.7和28.7％，GYB001T和副杆菌属JCM18682T之间的平均核苷酸同一性和数字DNA-DNA杂交值分别为86.6和27.7％，分别。基因组长6.57Mbp，G+C含量为43.3mol%。布鲁氏菌血琼脂（BBA）上的菌落呈圆形，凸面,光滑,灰色和小的大小。在胰蛋白酶大豆琼脂（TSA）上观察到生长，TSA+5%羊血和裸藻琼脂。在0-3％NaCl（w/v）的存在下和pH6.0-8.5下，在BBA上在18-42°C下生长。主要的极性脂质是磷脂酰乙醇胺和磷脂。菌株GYB001T中的主要脂肪酸是anteiso-C15：0和iso-C17：03-OH，主要的呼吸醌是甲基萘醌-10（MK-10）和MK-9。细胞壁含有内消旋二氨基庚二酸。考虑到这些表型特征和比较基因组分析，我们建议菌株GYB001T为李氏副杆菌的类型菌株。11月。(=KCTC25738T=KBN12P06525T=LMG32797T)。
