PDF(Pubmed)
Abstract：
Soybean sudden death syndrome (SDS) is a destructive disease that causes substantial yield losses in South and North America. Whereas four Fusarium species were identified as the causal agents, F. virguliforme is the primary SDS-causing pathogen in North America and it also contributes substantially to SDS in Argentina. In this study, we comparatively analyzed genome assemblies of four F. virguliforme strains and identified 29 informative microsatellite markers. Sixteen of the 29 markers were used to investigate the genetic diversity and population structure of this pathogen in a collection of 90 strains from Argentina and the USA. A total of 37 multilocus genotypes (MLGs) were identified, including 10 MLGs in Argentina and 26 in the USA. Only MLG2, the most dominant MLG, was found in both countries. Analyses with three different approaches showed that these MLGs could be grouped into three clusters. Cluster IA consisting of four MLGs exclusively from the USA has much higher genetic diversity than the other two clusters, suggesting that it may be the ancestral cluster although additional data are necessary to support this hypothesis. Clusters IB and II consisted of 13 and 21 MLGs, respectively. MLGs belonging to these two clusters were present in all four sampled states in Argentina and all five sampled states in the USA.
摘要：
大豆猝死综合症（SDS）是一种破坏性疾病，在南美和北美引起大量产量损失。尽管四种镰刀菌被确定为病原体，F.virguliforme是北美主要的引起SDS的病原体，在阿根廷也对SDS有很大贡献。在这项研究中,我们比较分析了4株F.virguliforme菌株的基因组组装，并鉴定了29个信息丰富的微卫星标记。在来自阿根廷和美国的90个菌株中，使用了29个标记中的16个来研究该病原体的遗传多样性和种群结构。共鉴定出37种多位点基因型(MLGs),包括阿根廷的10个MLG和美国的26个MLG。只有MLG2，最主要的MLG，在这两个国家都有发现。使用三种不同方法的分析表明，这些MLG可以分为三个集群。由四个完全来自美国的MLG组成的集群IA比其他两个集群具有更高的遗传多样性，这表明它可能是祖先集群，尽管需要额外的数据来支持这一假设。集群IB和II由13个和21个MLG组成，分别。属于这两个集群的MLG存在于阿根廷的所有四个采样州和美国的所有五个采样州。
